HiYield Plasmid Mini Kit 3.0
Product overview
Description
HiYield Plasmid Mini Kit 3.0 is specially designed for rapid isolation of plasmid or cosmid DNA from 1-4 ml of bacterial cultured cells. As high as 50 μg of high-quality plasmid DNA can be purified in less than 20 minutes and is ready for restriction digestion, ligation, PCR, and sequencing reaction.
No Phenol Extraction or alcohol precipitation is required in this protocol. In the process, clear and extra pure cell lysate with minimal genomic DNA and RNA contaminants can be obtained through the modified alkaline lysis method within the lysate will then bind to the glass fiber matrix equipped in the spin column. The contaminants are washed away with an ethanol-containing wash buffer. Finally, the purified plasmid DNA is eluted by a low salt elution buffer or distilled water. Typical yields of high-purity are 20-50 μg for high-copy number plasmids or 3~10 μg for low-copy number plasmids.
Features
- Low contaminants: glass fiber matrix
- High Yield: up to 50 μg
Samples
- 1-4 ml bacterial cultured cells
Applications
- Restriction enzyme digestion, library screening, ligation, PCR, transformation/sequencing reactions
Technical / Specifications
Store at
Specifications
| SYG011-001 (4 pcs) | FYG011-100 (100 pcs) | FYG011-300 (300 pcs) | |
| PN1 Buffer | 1 ml | 25 ml | 65 ml |
| PN2 Buffer | 1 ml | 25 ml | 65 ml |
| PN3 Buffer | 1.5 ml | 35 ml | 95 ml |
| W1 Buffer | 2 ml | 45 ml | 125 ml |
| W2 Buffer | 300 μl x2 | 15 ml | 25 ml x2 |
| Elution Buffer | 1 ml | 10 ml | 30 ml |
| RNase A (50 mg/ ml) | Added | 50 μl | 150 μl |
| PN Column | 4 pcs | 100 pcs | 300 pcs |
| Collection Tubes | 4 pcs | 100 pcs | 300 pcs |
| User Manual | 1 | 1 | 1 |
Support/ Documents
| Manual | |
 |