DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.
Features
Streptomycin resistant
High transformation efficiency
Applications
Suitable for genomic DNA cloning
Allow large inserts
Cloning of methylated cytosine or adenine containing DNA
