ECOS™ Sonic™ Competent Cells [BL21(DE3) Derived]
Strain: BL21(DE3) Derived
Description
ECOS™ competent cells is the fast transformation competent cells developed by Yeastern Biotech technology. Without additional gene
modification, the process takes less than 6 minutes to yield the high efficiency without the need of SOC recovery (with ampicillin).
ECOS™ Sonic competent cells is the derivative of BL21(DE3) strain.
It inherits the protein expression and fast-growing feature from its parent strain. The further deletion of endA and recA improve the
quality of plasmid DNA and enable the plasmid extraction in 3-6 hours growing.
modification, the process takes less than 6 minutes to yield the high efficiency without the need of SOC recovery (with ampicillin).
ECOS™ Sonic competent cells is the derivative of BL21(DE3) strain.
It inherits the protein expression and fast-growing feature from its parent strain. The further deletion of endA and recA improve the
quality of plasmid DNA and enable the plasmid extraction in 3-6 hours growing.
Genotype
F– ompT hsdSB (rB– ,mB– ) ΔrecA ΔendA gal dcm (DE3)
Features and Advantages
- High efficiency
- Hiyield and rapid plasmid production (only 4~6 hr needed)
- Direct plasmid extraction
Applications
- Fast protein screening
- Master cell bank
- Nucleic acid medicine
- Protein expression
Efficiency
>1 x 108 cfu/ μg Recommended for:
- Subcloning
- General cloning
Specification
Store at 
# FYE907-10VL (5 preps)
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 5 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE907-20VL (20 preps)
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 20 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| Manual | ECOS Card |
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