YEAtaq II DNA Polymerase

Description
YEAtaq II DNA Polymerase is a thermostable enzyme derived from Thermus aquaticus YT-1 strain.
The enzyme is in a recombinant form expressed in E. coli. It is able to with stand repeated heating to 95°C without significant loss of activity.
It possesses both 5’-3’ polymerase and exonuclease activity, and has no detectable 3’-5’ exonuclease activity.
It has a 3’ adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.
 
Features
• 1 kb/ 1 min
 
Applications
• Cloning and expression
• Mutation analysis
• Efficient & highly sensitive PCR use
 
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 72 °C.
 
Quality Control
• Nuclease activity is not detected after incubation of 1 μg λ/Hind III DNA with 5 units of HiFi DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
• The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed
by appropriate tests. A functional test is performed by PCR.
  

Store at

# FYT601-500U (500 units) 

YEAtaq II DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 1 ml
dNTPs Mix 200 μl (10 mM)

# FYT611-500U (500 units) 

HiFi DNA Polymerase 100 μl (5 U/μl)
10× Reaction Buffer 1 ml

Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 0.5% Tween 20, 1 mM DTT, 50% Glycerol, Stabilizers

10× Reaction Buffer*

15 mM MgCl2, Tris-HCl (pH 8.3), (NH4)2SO4, KCl

* The reaction buffer is supplied as a 10× concentrate and should be diluted before use.

Manual