ECOS™ Blue Competent Cells [XL1-Blue]

Product overview

Description
XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Features
  • A strain modified to be ideal for blue/white screening
  • Tetracycline resistant
Applications
  • Routine cloning application
  • Subcloning
  • cDNA library
  • Blue/white screening

Technical / Specifications

Store at

Genotype
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proABlacIqZDM15 Tn10 (TetR)]

 


Specifications

# FYE107-10VL (10 preps)
Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE107-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE108-10VL (10 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE108-80VL (80 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE109-10VL (10 preps)

Efficiency >2 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE109-80VL (80 preps)

Efficiency >2 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

# FYE107-96WL (96 preps)

Efficiency >1 x 108 cfu/μg
50 μl/well 96-wells

# FYE108-96WL (96 preps)

Efficiency >5 x 108 cfu/μg
50 μl/well 96-wells

# FYE109-96WL (96 preps)

Efficiency >1 x 109 cfu/μg
50 μl/well 96-wells

 

Support/ Documents

 

Manual ECOS Card