ECOS™ 101 Competent Cells [DH5α]
Product overview
Description
DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Technical / Specifications
Store at
Genotype
Specifications
# FYE678-10VL (10 preps)
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE678-80VL (80 preps)
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE607-10VL (10 preps)
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE607-80VL (80 preps)
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE608-10VL (10 preps)
| Efficiency >3 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE608-80VL (80 preps)
| Efficiency >3 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE609-10VL (10 preps)
| Efficiency >1 x 109 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE609-80VL (80 preps)
| Efficiency >1 x 109 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
# FYE607-96WL (96 preps)
| Efficiency >1 x 108 cfu/μg | |
| 50 μl/well | 96 wells |
# FYE608-96WL (96 preps)
| Efficiency >3 x 108 cfu/μg | |
| 50 μl/well | 96 wells |
# FYE609-96WL (96 preps)
| Efficiency >1 x 109 cfu/μg | |
| 50 μl/well | 96 wells |
Support/ Documents
| Manual | ECOS Card |
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