ECOS™ Competent Cells

The fastest transforming cells worldwide-

1 minute transformation

With “ECOS™” standing for “E. Coli One-Step”, and its reverse “SOCE” standing for “SOC Eliminated”,  the name itself speaks for the advantages.
1 minute, 1 step high-efficiency transformation and no SOC recovery step required, truly a design to save your time.

Choose a strain

Don't know which strain to use? LOOK HERE!

Selection Guide for ECOS Competent Cells

Mutations and Respective Strains

DH5α

With its high efficiency, it is suitable for cloning large plasmids and cDNA library construction, also allowing for blue-white screening

Use for

Cloning / Subcloning 

Scale-up application

Blue/white screening

ECOS™ X Competent Cells

[DH5α]

 

Super High Efficiency

~5 x 109 cfu/μg

One step, one tube, finish in 6 minutes !

ECOS™ 101 Competent Cells

[DH5α]

High Efficiency

One step, one tube, finish in 6 minutes !

BL21(DE3)

Lack of Lon and Omp proteases allows for high-level protein expression

Use for

IPTG induced gene expression of  toxic/non-toxic protein expression using T7-based vectors

Subcloning

ECOS™ 21 Competent Cells [BL21(DE3)]

 

High Levels of Protein Expression

One step, one tube, finish in 6 minutes !

ECOS™ Sonic™ Competent Cells [BL21(DE3) Derived]

High Efficiency >108 cfu/ug

One step, one tube, finish in 6 minutes !  

JM109

The fastest growing strain; colonies show up about 8 hours. Also carries the F’episome, which allows for blue/white screening

Use for

Urgent needs cloning needs

Preparation of ssDNA

Gene library construction

Subcloning

ECOS™ 9-5 Competent Cells [JM109]

Rapid Growing 

One step, one tube, finish in 6 minutes !

DH10B

A MC1061 derivative which is Streptomycin resistant and can prevent DNA methylation

Designed to propagate large inserts in DNA library cloning

Use for

Genomic library cloning

Large inserts

Cloning of methylated cytosine or adenine containing DNA

Blue/white screening

ECOS™ 10B Competent Cells [DH10B]

Allow Large Inserts

One step, one tube, finish in 6 minutes !

XL-Blue

A Tetracycline resistant strain modified to be ideal for blue/white screening

Use for

Blue/white screening

Cloning / Subcloning

cDNA library

ECOS™ Blue Competent Cells [XL1-Blue]

Ideal for Blue/white Screening 

One step, one tube, finish in 6 minutes !

GM2163

A Chloramphenicol resistant K12 strain deficient in both dam and dcm genes, for use with Dam or Dcm-sensitive restriction enzymes

Use for 

Plasmids free of Dam and Dcm methylations

ECOS™ 2163 Competent Cells [GM2163]

No Methylations

One step, one tube, finish in 6 minutes !

Transformation Protocol

Special Tips

Patented Technology
Yeastern Biotech Co., Ltd. owns the patent of ECOS™ technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No. US 6,864,088; GB 2383582; I 229696; FR 2832727, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS™ chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS™ protocol or its similar version, please contact our headquarters in Taiwan for licensing.
Quality Control
Each lot of competent cells has to pass three quality control tests before shipping:
  1. Efficiency test: each batch of ECOS™ should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.
  2. Contamination test: competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.
  3. A-complementation test: this test is performed for all ECOS™ competent cells except for ECOS™ 21(DE3) and ECOS™ 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%