{"id":6958,"date":"2024-07-23T14:04:43","date_gmt":"2024-07-23T06:04:43","guid":{"rendered":"https:\/\/yeastern.com\/?page_id=6958"},"modified":"2024-07-23T15:38:08","modified_gmt":"2024-07-23T07:38:08","slug":"genomic-dna-extraction-kit-cultured-cell-2-0-2","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=6958","title":{"rendered":"Genomic DNA Extraction Kit (Bacteria\/Fungi) 2.0"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.16″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_menu menu_id=”76″ active_link_color=”RGBA(255,255,255,0)” _builder_version=”4.25.1″ menu_font=”|700|||||||” menu_text_color=”#575757″ menu_font_size=”15px” background_enable_color=”off” width=”76%” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”|32px||||” menu_text_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.25.1″ title_font=”|600|||||||” custom_margin=”||26px|||” global_colors_info=”{}”][\/et_pb_post_title][et_pb_toggle title=”Product overview” _builder_version=”4.16″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

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Description<\/strong><\/h6>\n<\/div>\n
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The Genomic DNA Kit (Bacteria\/Fungi) provides an efficient method for purifying total DNA from bacteria or fungi. Chaotropic salt is used to lyse cells and degrade proteins, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using the Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The entire procedure can be completed in 40 minutes without phenol\/chloroform extraction or alcohol precipitation, with an average DNA yield of 60 \u03bcg from 10^9 bacteria cells or 10^8 fungi cells. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.<\/p>\n

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Features<\/strong><\/h6>\n
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  • Low contaminants: glass fiber matrix<\/li>\n
  • High Yield: up to 50 \u03bcg<\/li>\n<\/ul>\n
    Samples<\/strong><\/h6>\n
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    • 10^9 bacteria cells<\/li>\n
    • 10^8 fungi cells<\/li>\n<\/ul>\n
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      Applications<\/strong><\/h6>\n
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      • PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

        [\/et_pb_toggle][et_pb_toggle title=”Technical \/ Specifications” _builder_version=”4.27.0″ title_level=”h4″ title_font=”|600|||||||” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

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        Store at<\/strong><\/span><\/h6>\n
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        Specifications<\/strong><\/h6>\n
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        <\/div>\n<\/div>\n<\/div>\n<\/div>\n
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        <\/td>\nSYG115-001 (4 pcs)<\/strong><\/td>\nSYG115-100P (100 pcs)<\/strong><\/td>\n<\/tr>\n
        BF1 Buffer<\/td>\n2 ml<\/td>\n20 ml<\/td>\n<\/tr>\n
        BF2 Buffer<\/td>\n1.5 ml<\/td>\n35 ml<\/td>\n<\/tr>\n
        BF3 Buffer<\/td>\n2 ml<\/td>\n45 ml<\/td>\n<\/tr>\n
        W1 Buffer<\/td>\n2 ml<\/td>\n45 ml<\/td>\n<\/tr>\n
        W2 Buffer<\/td>\n300 \u03bcl x2<\/td>\n15 ml<\/td>\n<\/tr>\n
        Elution Buffer<\/td>\n1 ml<\/td>\n10 ml<\/td>\n<\/tr>\n
        BF Column<\/td>\n4 pcs<\/td>\n100 pcs<\/td>\n<\/tr>\n
        Collection Tubes<\/td>\n4 pcs<\/td>\n100 pcs<\/td>\n<\/tr>\n
        User Manual<\/td>\n1<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

        [\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n

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        Manual<\/span><\/strong><\/td>\n<\/td>\n<\/tr>\n
        \"\"<\/a><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

        [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

        Description The Genomic DNA Kit (Bacteria\/Fungi) provid […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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        \r\n\r\nStrain: DH5\u03b1<\/b>\r\n\r\n<\/div>\r\n
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        Description<\/div>\r\n
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        DH5\u03b1 is the most popular\u00a0E. coli<\/em>\u00a0strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries\u00a0rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries\u00a0end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/div>\r\n<\/div>\r\n
        Genotype<\/div>\r\n
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        end<\/em>A1\u00a0rec<\/em>A1\u00a0rel<\/em>A1\u00a0gyr<\/em>A96\u00a0hsd<\/em>R17(rk-<\/sup>, mk+<\/sup><\/em>)\u00a0pho<\/em>A\u00a0sup<\/em>E44\u00a0thi<\/em>-1 \u0394(lac<\/em>ZYA-arg<\/em>F)U169 \u03a680 \u0394(lac<\/em>Z)M15 F-<\/sup><\/div>\r\n<\/div>\r\n
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        Features<\/div>\r\n
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        Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.<\/div>\r\n<\/div>\r\n
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        Applications<\/div>\r\n
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