{"id":6939,"date":"2024-07-22T13:42:52","date_gmt":"2024-07-22T05:42:52","guid":{"rendered":"https:\/\/yeastern.com\/?page_id=6939"},"modified":"2024-07-22T15:40:21","modified_gmt":"2024-07-22T07:40:21","slug":"hiyield-plasmid-mini-kit-3-0","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=6939","title":{"rendered":"HiYield Plasmid Mini Kit 3.0"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.16″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_menu menu_id=”76″ active_link_color=”RGBA(255,255,255,0)” _builder_version=”4.25.1″ menu_font=”|700|||||||” menu_text_color=”#575757″ menu_font_size=”15px” background_enable_color=”off” width=”76%” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”|32px||||” menu_text_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.25.1″ title_font=”|600|||||||” custom_margin=”||26px|||” global_colors_info=”{}”][\/et_pb_post_title][et_pb_toggle title=”Product overview” _builder_version=”4.16″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

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Description<\/strong><\/h6>\n<\/div>\n
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HiYield Plasmid Mini Kit 3.0 is specially designed for rapid isolation of plasmid or cosmid DNA from 1-4 ml of bacterial cultured cells. As high as 50 \u03bcg of high-quality plasmid DNA can be purified in less than 20 minutes and is ready for restriction digestion, ligation, PCR, and sequencing reaction.<\/p>\n

No Phenol Extraction or alcohol precipitation is required in this protocol. In the process, clear and extra pure cell lysate with minimal genomic DNA and RNA contaminants can be obtained through the modified alkaline lysis method within the lysate will then bind to the glass fiber matrix equipped in the spin column. The contaminants are washed away with an ethanol-containing wash buffer. Finally, the purified plasmid DNA is eluted by a low salt elution buffer or distilled water. Typical yields of high-purity are 20-50 \u03bcg for high-copy number plasmids or 3~10 \u03bcg for low-copy number plasmids.<\/p>\n

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Features<\/strong><\/h6>\n
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  • Low contaminants: glass fiber matrix<\/li>\n
  • High Yield: up to 50 \u03bcg<\/li>\n<\/ul>\n
    Samples<\/strong><\/h6>\n
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    • 1-4 ml bacterial cultured cells<\/li>\n<\/ul>\n
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      Applications<\/strong><\/h6>\n
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      • Restriction enzyme digestion, library screening, ligation, PCR, transformation\/sequencing reactions<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

        [\/et_pb_toggle][et_pb_toggle title=”Technical \/ Specifications” _builder_version=”4.27.0″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

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        \u00a0Store at<\/strong><\/span><\/h6>\n
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        Specifications<\/strong><\/h6>\n
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        <\/div>\n<\/div>\n<\/div>\n<\/div>\n
        \n\n\n\n\n\n\n\n\n\n\n\n\n\n
        <\/td>\nSYG011-001 (4 pcs)<\/strong><\/td>\nFYG011-100 (100 pcs)<\/strong><\/td>\nFYG011-300 (300 pcs)<\/strong><\/td>\n<\/tr>\n
        PN1 Buffer<\/td>\n1 ml<\/td>\n25 ml<\/td>\n65 ml<\/td>\n<\/tr>\n
        PN2 Buffer<\/td>\n1 ml<\/td>\n25 ml<\/td>\n65 ml<\/td>\n<\/tr>\n
        PN3 Buffer<\/td>\n1.5 ml<\/td>\n35 ml<\/td>\n95 ml<\/td>\n<\/tr>\n
        W1 Buffer<\/td>\n2 ml<\/td>\n45 ml<\/td>\n125 ml<\/td>\n<\/tr>\n
        W2 Buffer<\/td>\n300 \u03bcl x2<\/td>\n15 ml<\/td>\n25 ml x2<\/td>\n<\/tr>\n
        Elution Buffer<\/td>\n1 ml<\/td>\n10 ml<\/td>\n30 ml<\/td>\n<\/tr>\n
        RNase A (50 mg\/ ml)<\/td>\nAdded<\/td>\n50 \u03bcl<\/td>\n150 \u03bcl<\/td>\n<\/tr>\n
        PN Column<\/td>\n4 pcs<\/td>\n100 pcs<\/td>\n300 pcs<\/td>\n<\/tr>\n
        Collection Tubes<\/td>\n4 pcs<\/td>\n100 pcs<\/td>\n300 pcs<\/td>\n<\/tr>\n
        User Manual<\/td>\n1<\/td>\n1<\/td>\n1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

        [\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n

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        Manual<\/span><\/strong><\/td>\n<\/td>\n<\/tr>\n
        \"\"<\/a><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

        [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

        Description HiYield Plasmid Mini Kit 3.0 is specially d […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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        \r\n\r\nStrain: DH5\u03b1<\/b>\r\n\r\n<\/div>\r\n
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        Description<\/div>\r\n
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        DH5\u03b1 is the most popular\u00a0E. coli<\/em>\u00a0strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries\u00a0rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries\u00a0end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/div>\r\n<\/div>\r\n
        Genotype<\/div>\r\n
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        end<\/em>A1\u00a0rec<\/em>A1\u00a0rel<\/em>A1\u00a0gyr<\/em>A96\u00a0hsd<\/em>R17(rk-<\/sup>, mk+<\/sup><\/em>)\u00a0pho<\/em>A\u00a0sup<\/em>E44\u00a0thi<\/em>-1 \u0394(lac<\/em>ZYA-arg<\/em>F)U169 \u03a680 \u0394(lac<\/em>Z)M15 F-<\/sup><\/div>\r\n<\/div>\r\n
        <\/div>\r\n
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        Features<\/div>\r\n
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        Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.<\/div>\r\n<\/div>\r\n
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        Applications<\/div>\r\n
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