{"id":6921,"date":"2024-07-22T11:58:36","date_gmt":"2024-07-22T03:58:36","guid":{"rendered":"https:\/\/yeastern.com\/?page_id=6921"},"modified":"2024-07-22T13:16:57","modified_gmt":"2024-07-22T05:16:57","slug":"etboutnucleic-acid-staining-solution-2-0","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=6921","title":{"rendered":"EtB\u201cOut\u201dNucleic Acid Staining Solution 2.0"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.16″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_menu menu_id=”76″ active_link_color=”RGBA(255,255,255,0)” _builder_version=”4.25.1″ menu_font=”|700|||||||” menu_text_color=”#575757″ menu_font_size=”15px” background_enable_color=”off” width=”76%” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”|32px||||” menu_text_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.25.1″ title_font=”|600|||||||” custom_margin=”||26px|||” global_colors_info=”{}”][\/et_pb_post_title][et_pb_toggle title=”Product overview” _builder_version=”4.27.0″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

\n

Nucleic acids detection (dsDNA and ssRNA) within agarose gel after
electrophoresis under UV illumination<\/p>\n

\n
\n
Description<\/strong><\/h6>\n<\/div>\n
\n

EtB\u201cOut\u201d Nucleic Acid Staining Solution 2.0 aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels. EtBr
has long been known as a strong mutagen, however EtB\u201cOut\u201d causes only neglectable mutations in the Ames test.
The sensitivity of EtB\u201cOut\u201d is identical to that of EtBr. Under UV light, EtB\u201cOut\u201d emits green fluorescence when bound to DNA or RNA. EtB\u201cOut\u201d can be
excited at 290 nm and 490 nm. The fluorescence emission peak of EtB\u201cOut\u201d when bound to DNA is at 537 nm.<\/p>\n<\/div>\n

<\/strong><\/h6>\n
Features<\/strong><\/h6>\n
    \n
  • \uf06c Economic: use only 5 \u03bcl in 100 ml of agarose gel<\/li>\n
  • \u00a0 \u00a0Safe: non-mutagenic, non-toxic, non-carcinogenic<\/li>\n
  • \u00a0 \u00a0Green: no hazardous waste<\/li>\n
  • \u00a0 \u00a0Sensitive: sensitivity is comparable and even better than EtBr, DNA concentration as low as 5 ng can be detected<\/li>\n<\/ul>\n
    <\/strong><\/h6>\n
    <\/strong><\/h6>\n
    Applications<\/strong><\/h6>\n
    \n
    \n
      \n
    • Nucleic acids detection (dsDNA and ssRNA) within agarose gel after electrophoresis under UV illumination<\/li>\n<\/ul>\n

       <\/p>\n

      \"\"\"\"<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

      [\/et_pb_toggle][et_pb_toggle title=”Technical \/ Specifications” _builder_version=”4.27.0″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

      \n
      \n
      \n
      tore at<\/strong><\/span><\/h6>\n
      \"\"<\/strong><\/span><\/h6>\n
      <\/h6>\n<\/div>\n
      <\/strong><\/h6>\n
      Specifications<\/strong><\/h6>\n
      \n
      <\/div>\n<\/div>\n<\/div>\n<\/div>\n
      \n

      # FYD010-200P<\/b> (20,000x, 1 mL)<\/span><\/p>\n

      \n\n\n\n\n\n
      Loading volume: 5 <\/b><\/b>\u03bcL\/ well<\/strong><\/td>\n<\/tr>\n
      20,000x, 1 mL<\/td>\n<\/td>\n<\/tr>\n
      <\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
      <\/div>\n
      <\/div>\n<\/div>\n

      [\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n

      <\/div>\n\n\n\n\n
      Manual<\/span><\/strong><\/td>\n<\/td>\n<\/tr>\n
      \"\"<\/a><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

      Nucleic acids detection (dsDNA and ssRNA) within agaros […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

      \r\n
      \r\n
      \r\n
      \r\n
      \r\n
      \r\n
      \r\n
      \r\n
      \r\n\r\nStrain: DH5\u03b1<\/b>\r\n\r\n<\/div>\r\n
      \r\n
      Description<\/div>\r\n
      \r\n
      DH5\u03b1 is the most popular\u00a0E. coli<\/em>\u00a0strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries\u00a0rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries\u00a0end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/div>\r\n<\/div>\r\n
      Genotype<\/div>\r\n
      \r\n
      end<\/em>A1\u00a0rec<\/em>A1\u00a0rel<\/em>A1\u00a0gyr<\/em>A96\u00a0hsd<\/em>R17(rk-<\/sup>, mk+<\/sup><\/em>)\u00a0pho<\/em>A\u00a0sup<\/em>E44\u00a0thi<\/em>-1 \u0394(lac<\/em>ZYA-arg<\/em>F)U169 \u03a680 \u0394(lac<\/em>Z)M15 F-<\/sup><\/div>\r\n<\/div>\r\n
      <\/div>\r\n
      <\/div>\r\n
      Features<\/div>\r\n
      \r\n
      Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.<\/div>\r\n<\/div>\r\n
      <\/div>\r\n
      Applications<\/div>\r\n
      \r\n
      \r\n