{"id":6907,"date":"2024-06-04T16:45:14","date_gmt":"2024-06-04T08:45:14","guid":{"rendered":"https:\/\/yeastern.com\/?page_id=6907"},"modified":"2024-06-04T16:45:44","modified_gmt":"2024-06-04T08:45:44","slug":"1-kb-yea-ladder-dna-marker-iv-ready-to-use","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=6907","title":{"rendered":"1 kb YEA Ladder DNA Marker IV (Ready-to-Use)"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”0px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ _builder_version=”4.16″ width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px|0px|0px|0px|false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_menu menu_id=”76″ active_link_color=”RGBA(255,255,255,0)” _builder_version=”4.25.1″ menu_font=”|700|||||||” menu_text_color=”#575757″ menu_font_size=”15px” background_enable_color=”off” width=”76%” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” custom_padding=”|32px||||” menu_text_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.25.1″ title_font=”|600|||||||” custom_margin=”||26px|||” global_colors_info=”{}”][\/et_pb_post_title][et_pb_toggle title=”Product overview” _builder_version=”4.16″ title_level=”h4″ title_font=”|800|||||||” closed_title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

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Description<\/strong><\/h6>\n<\/div>\n
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The Yeastern Biotech’s 1 kb YEA Ladder DNA Marker IV (FYD011-1ML) contains 13 discrete fragments ranging from 250 bp to 12 kb.
The wide range allows you to identify target DNA to the greatest extent. It is supplied in a ready-to-use format containing blue tracking dyes, and mixed at varied concentrations.<\/p>\n<\/div>\n

For best results, load 3 \u03bcL of the marker per well. There are two fragments, 1.5 kb and 4 kb, as the reference bands which are approximately 90 ng each for these two bands and\u00a0 30 ng for other bands if load 3 \u03bcL per well. It allows sizing and concentration estimation of DNA fragments on agarose gel generation by PCR or restriction digestion.<\/h6>\n
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Features<\/strong><\/h6>\n
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  • Broad range<\/li>\n
  • High concentration<\/li>\n<\/ul>\n
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    Applications<\/strong><\/h6>\n
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    • Locating DNA of interest<\/li>\n
    • DNA quantification<\/li>\n
    • DNA tracking<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

      [\/et_pb_toggle][et_pb_toggle title=”Technical \/ Specifications” _builder_version=”4.25.1″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n

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      Store at<\/strong><\/span><\/h6>\n

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      Specifications<\/strong><\/h6>\n
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      # FYD011-1ML<\/b> (1 mL)<\/span><\/p>\n

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      Loading volume: 2~3 <\/b><\/b>\u03bcL\/ well<\/strong><\/td>\n<\/tr>\n
      170 ng\/\u03bcL, 1 mL<\/td>\n<\/td>\n<\/tr>\n
      5x DNA Loading dye, 1 mL<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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      [\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n

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      Manual<\/span><\/strong><\/td>\n<\/td>\n<\/tr>\n
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      [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

      Description The Yeastern Biotech’s 1 kb YEA Ladder DNA […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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      \r\n\r\nStrain: DH5\u03b1<\/b>\r\n\r\n<\/div>\r\n
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      Description<\/div>\r\n
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      DH5\u03b1 is the most popular\u00a0E. coli<\/em>\u00a0strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries\u00a0rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries\u00a0end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/div>\r\n<\/div>\r\n
      Genotype<\/div>\r\n
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      end<\/em>A1\u00a0rec<\/em>A1\u00a0rel<\/em>A1\u00a0gyr<\/em>A96\u00a0hsd<\/em>R17(rk-<\/sup>, mk+<\/sup><\/em>)\u00a0pho<\/em>A\u00a0sup<\/em>E44\u00a0thi<\/em>-1 \u0394(lac<\/em>ZYA-arg<\/em>F)U169 \u03a680 \u0394(lac<\/em>Z)M15 F-<\/sup><\/div>\r\n<\/div>\r\n
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      Features<\/div>\r\n
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      Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.<\/div>\r\n<\/div>\r\n
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      Applications<\/div>\r\n
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