{"id":6763,"date":"2024-05-30T16:39:44","date_gmt":"2024-05-30T08:39:44","guid":{"rendered":"https:\/\/yeastern.com\/?page_id=6763"},"modified":"2024-05-31T15:37:16","modified_gmt":"2024-05-31T07:37:16","slug":"hifi-dna-polymerase-2","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=6763","title":{"rendered":"YEAtaq II DNA Polymerase"},"content":{"rendered":"

[et_pb_section fb_built=”1″ admin_label=”section” _builder_version=”4.16″ global_colors_info=”{}”][et_pb_row column_structure=”1_4,3_4″ use_custom_gutter=”on” gutter_width=”1″ admin_label=”row” _builder_version=”4.25.1″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” width=”85%” max_width=”2560px” module_alignment=”center” custom_padding=”30px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_menu menu_id=”76″ _builder_version=”4.25.1″ _module_preset=”default” menu_font=”|700|||||||” width=”98.1%” custom_padding=”|206px||||” hover_enabled=”0″ menu_text_shadow_style=”preset1″ global_colors_info=”{}” sticky_enabled=”0″ menu_font_size=”15px”][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.25.1″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” title_font=”Arial|600|||||||” sticky_enabled=”0″][\/et_pb_post_title][et_pb_text admin_label=”Text” _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”]<\/p>\n

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Description<\/strong><\/div>\n
YEAtaq II DNA Polymerase is a thermostable enzyme derived from Thermus aquaticus YT-1 strain.<\/div>\n
The enzyme is in a recombinant form expressed in E. coli. It is able to with stand repeated heating to 95\u00b0C without significant loss of activity.<\/div>\n
It possesses both 5\u2019-3\u2019 polymerase and exonuclease activity, and has no detectable 3\u2019-5\u2019 exonuclease activity.<\/div>\n
It has a 3\u2019 adenylation activity. Thus, the PCR products can be used directly in TA-cloning procedures.<\/div>\n
\u00a0<\/div>\n
Features<\/strong><\/div>\n
\u2022 1 kb\/ 1 min<\/div>\n
\u00a0<\/strong><\/div>\n
Applications<\/strong><\/div>\n
\u2022 Cloning and expression
\u2022 Mutation analysis
\u2022 Efficient & highly sensitive PCR use<\/div>\n
\u00a0<\/div>\n
Unit Definition<\/strong>
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 72 \u00b0C.<\/div>\n
\u00a0<\/strong><\/div>\n
Quality Control<\/strong><\/div>\n
\u2022 Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb\/Hind III DNA with 5 units of HiFi DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C.
\u2022 The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed
by appropriate tests. A functional test is performed by PCR.<\/div>\n
\u00a0\u00a0<\/span><\/div>\n<\/div>\n<\/div>\n
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Store at<\/span><\/p>\n

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# FYT601-500U<\/b>\u00a0(500 units)\u00a0<\/span><\/p>\n

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YEAtaq II DNA Polymerase<\/td>\n100 \u03bcl (5 U\/\u03bcl)<\/td>\n<\/tr>\n
10\u00d7 Reaction Buffer<\/td>\n1 ml<\/td>\n<\/tr>\n
dNTPs Mix<\/td>\n200 \u03bcl (10 mM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

# FYT611-500U<\/b>\u00a0(500 units)\u00a0<\/span><\/p>\n

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HiFi DNA Polymerase<\/td>\n100 \u03bcl (5 U\/\u03bcl)<\/td>\n<\/tr>\n
10\u00d7 Reaction Buffer<\/td>\n1 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n

Storage Buffer<\/b><\/span><\/p>\n

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20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 0.5% Tween 20, 1 mM DTT, 50% Glycerol, Stabilizers<\/p>\n<\/div>\n

10\u00d7 Reaction Buffer*<\/b><\/span><\/p>\n

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15 mM MgCl2<\/sub>, Tris-HCl (pH 8.3), (NH4<\/sub>)2<\/sub>SO4<\/sub>, KCl<\/p>\n

* The reaction buffer is supplied as a 10\u00d7\u00a0concentrate and should be diluted before use.<\/p>\n<\/div>\n<\/div>\n\n\n\n\n
Manual<\/td>\n<\/tr>\n
\u00a0\u00a0<\/a>\"\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Description YEAtaq II DNA Polymerase is a thermostable […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

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Description<\/strong><\/div>\r\n
HiFi DNA polymerase is ideal for amplification of DNA fragments that require high-fidelity. High fidelity is achieved by an optimal blend of high-performance YEAtaq DNA polymerase and Accu DNA polymerase, a Pyrococcus proofreading (3'\u21925' exonuclease activity ) enzyme. This formulation achieves greater yields with higher fidelity than standard DNA polymerases such as Taq. HiFi is a quality choice for sensitive applications. Because of the presence of Accu DNA polymerase in the blend, dUTP, dITP and primers containing these nucleotides should not be used in PCR because they hinder DNA synthesis. HiFi DNA polymerase is offered in two different packages, one with dNTPs mix and the other without. HiFi DNA Polymerase is also available in 2\u00d7 ready-to-use premix format with a green dye.<\/div>\r\n
\u00a0<\/div>\r\n
Features<\/strong><\/div>\r\n
\u2022 High Efficiency, as fast as 1 kb\/ 5 secs.<\/div>\r\n
\u2022 Robust amplification with minimal optimization.
\u2022 High yields of PCR products.
\u2022 Higher sensitivity compared to conventional Taq DNA Polymerase.
\u2022 Amplification of long targets up to 10 kb from genomic DNA.
\u2022 Generates 3\u2019-dA overhangs.
\u2022 Incorporates modified nucleotides.
\u2022 The error rate of HiFi DNA Polymerase in PCR is 8.3\u00d710-6<\/sup> errors per nucleotide per cycle.<\/div>\r\n
\u00a0<\/strong><\/div>\r\n
Applications<\/strong><\/div>\r\n
\u2022 Cloning and expression
\u2022 Blunt end amplification products
\u2022 Mutation analysis
\u2022 Efficient & highly sensitive PCR use
\u2022 Routine PCR amplification of DNA fragments up to 10 kb from genomic DNA
\u2022 RT-PCR<\/div>\r\n
\u00a0<\/div>\r\n
Unit Definition<\/strong>
One unit is defined as the amount of enzyme that will incorporate 10 nmol of
dNTPs into an acid-insoluble material in 30 minutes at 72 \u00b0C.<\/div>\r\n
\u00a0<\/strong><\/div>\r\n
Quality Control<\/strong><\/div>\r\n
\u2022 Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb\/Hind III DNA with 5 units of HiFi DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C.
\u2022 The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed
by appropriate tests. A functional test is performed by PCR.<\/div>\r\n
\u00a0<\/div>\r\n
Patterns\/Disclaimer<\/strong>
Some applications in which this product can be used may be covered by
patents issued and applicable in certain countries. Because purchase of this
the product does not include a license to perform any patented application, users
of this product may be required to obtain a patent license depending upon the
particular application and country in which the product is used.<\/div>\r\n<\/div>\r\n<\/div>\r\n
\u00a0<\/div>\r\n
Result<\/strong><\/div>\r\n
\u00a0\"\"<\/div><\/div>\r\n<\/div><\/div><\/div>
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Specification<\/div>\r\n
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Store at<\/span><\/p>\r\n

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# FYT003-500U<\/b>\u00a0(500 units)\u00a0<\/span><\/p>\r\n

\r\n\r\n\r\n\r\n\r\n\r\n
HiFi DNA Polymerase<\/td>\r\n100 \u03bcl (5 U\/\u03bcl)<\/td>\r\n<\/tr>\r\n
10\u00d7 Reaction Buffer<\/td>\r\n2 ml<\/td>\r\n<\/tr>\r\n
dNTPs Mix<\/td>\r\n200 \u03bcl (10 mM)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n

# FYT033-500U<\/b>\u00a0(500 units)\u00a0<\/span><\/p>\r\n

\r\n\r\n\r\n\r\n\r\n
HiFi DNA Polymerase<\/td>\r\n100 \u03bcl (5 U\/\u03bcl)<\/td>\r\n<\/tr>\r\n
10\u00d7 Reaction Buffer<\/td>\r\n2 ml<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n

Storage Buffer<\/b><\/span><\/p>\r\n

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50 mM Tris-HCl (pH 9.0), 100 mM NaCl, 0.1 mM EDTA, 1% Triton X-100, 5 mM DTT, 50% Glycerol, Stabilizers<\/p>\r\n<\/div>\r\n

10\u00d7 Reaction Buffer*<\/b><\/span><\/p>\r\n

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100 mM KCl, 20 mM MgSO4<\/sub>\u00b77H2<\/sub>O, 200 mM Tris-HCl (pH 8.8), 1% Triton X-100, 100 mM (NH4<\/sub>)2<\/sub>SO4<\/sub>, 1 mg\/ml BSA<\/p>\r\n

* The reaction buffer is supplied as a 10\u00d7\u00a0concentrate and should be diluted before use.<\/p>\r\n<\/div>\r\n<\/div>\r\n\r\n\r\n\r\n\r\n
Manual<\/td>\r\n<\/tr>\r\n
\u00a0\u00a0<\/a>\"\"<\/a><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table><\/div>\r\n<\/div><\/div><\/div><\/div><\/div>","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/6763"}],"collection":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6763"}],"version-history":[{"count":20,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/6763\/revisions"}],"predecessor-version":[{"id":6853,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/6763\/revisions\/6853"}],"wp:attachment":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}