{"id":1949,"date":"2017-07-25T14:25:22","date_gmt":"2017-07-25T06:25:22","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1949"},"modified":"2024-05-31T16:38:37","modified_gmt":"2024-05-31T08:38:37","slug":"tatm-expression-kit","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1949","title":{"rendered":"T&#038;A Expression Kit"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;section&#8221; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_4,3_4&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; width=&#8221;85%&#8221; max_width=&#8221;2560px&#8221; module_alignment=&#8221;center&#8221; custom_padding=&#8221;30px|0px|0px|0px|false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_menu menu_id=&#8221;76&#8243; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.25.1&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#474747&#8243; menu_font_size=&#8221;15px&#8221; width=&#8221;76%&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; menu_text_shadow_style=&#8221;preset1&#8243; sticky_enabled=&#8221;0&#8243;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; _builder_version=&#8221;4.25.1&#8243; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; title_font=&#8221;|700|||||||&#8221; sticky_enabled=&#8221;0&#8243;][\/et_pb_post_title][et_pb_text _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-vector.jpg\" width=\"732\" height=\"956\" alt=\"\" class=\"wp-image-2876 alignnone size-full\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-vector.jpg 3673w, https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-vector-768x1002.jpg 768w\" sizes=\"(max-width: 732px) 100vw, 732px\" \/><\/p>\n<p>[\/et_pb_text][et_pb_toggle title=&#8221;Product overview&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|800|||||||&#8221; closed_title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><strong>Description<\/strong><\/h6>\n<div id=\"pgc-1949-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1949-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div>\n<p>The T&amp;A\u2122 Expression Kit (C-terminal) (FYC201-10P) contains the T&amp;A\u2122 Expression Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3\u2019-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS\u2122).<\/p>\n<p>Use with <a href=\"https:\/\/yeastern.com\/?page_id=3953\">ECOS\u2122 Sonic\u2122 competent cells<\/a> for optimal performance!<\/p>\n<\/div>\n<div class=\"all_font\">\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Features<\/strong><\/h6>\n<div>\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div id=\"pgc-1949-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1949-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<ul>\n<li>Clone and\u00a0 express proteins in one step (use with <a href=\"https:\/\/yeastern.com\/?page_id=3953\">ECOS\u2122 Sonic\u2122<\/a>)<\/li>\n<li>5 minute ligation<\/li>\n<li>Bacteriophage T7 promoter for high-level expression; primers sites incuded for sequencing convenience<\/li>\n<li>C-terminal 6x His tag for detection or purification<\/li>\n<li>High transformation efficiency<\/li>\n<li>Two types of ligation buffers provided for your convenience<\/li>\n<li>Ampicillin markers for antibiotic selection<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div><strong><\/strong><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Applications<\/strong><\/h6>\n<div id=\"pgc-1949-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1949-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div><\/div>\n<div class=\"all_font\">\n<div>\n<p><b><\/b><\/p>\n<ul>\n<li>Gene construction and protein expression.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Specifications&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><span style=\"color: #000000;\"><strong>Store at<\/strong><\/span><\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2357\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\" alt=\"\" width=\"114\" height=\"84\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-230x170.png 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C.png 327w\" sizes=\"(max-width: 114px) 100vw, 114px\" \/><\/p>\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><\/h6>\n<\/div>\n<div>\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Specifications<\/strong><\/h6>\n<div>\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div id=\"pgc-1949-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1949-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p><strong>#FYC201-10P (10 preps)<\/strong><\/p>\n<p><strong>T&amp;A<span style=\"font-size: xx-small;\"><sup>TM<\/sup><\/span> Expression Kit\u00a0 (C-terminal) <\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"322\">T&amp;A Expression Vector\u00a0 (C-terminal)<\/td>\n<td width=\"183\">20 \u03bcl (25 ng\/\u03bcl)<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">Control Insert DNA (C-terminal)<\/td>\n<td width=\"183\">10 \u03bcl (10 ng\/\u03bcl)<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">yT4 DNA Ligase (2U)<\/td>\n<td width=\"183\">10 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">10X Ligation Buffer A<\/td>\n<td width=\"183\">50 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">10X Ligation Buffer B<\/td>\n<td width=\"183\">50 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">Primer T7 promoter<\/td>\n<td width=\"183\">50 \u03bcl (10\u03bcM)<\/td>\n<\/tr>\n<tr>\n<td width=\"322\">Primer T7 terminator<\/td>\n<td width=\"183\">50 \u03bcl (10\u03bcM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>#FYC211-10P (10 preps) <\/strong><\/p>\n<p><strong>T&amp;A<span style=\"font-size: xx-small;\"><sup>TM<\/sup><\/span> Expression Vector (C-terminal)<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"346\">T&amp;A Expression Vector\u00a0 (C-terminal)<\/td>\n<td width=\"151\">20 \u03bcl (25 ng\/\u03bcl)<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">Control Insert DNA (C-terminal)<\/td>\n<td width=\"151\">10 \u03bcl (10 ng\/\u03bcl)<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">Primer T7 promoter<\/td>\n<td width=\"151\">50 \u03bcl (10\u03bcM)<\/td>\n<\/tr>\n<tr>\n<td width=\"346\">Primer T7 terminator<\/td>\n<td width=\"151\">50 \u03bcl (10\u03bcM)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Technical&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\"><\/div>\n<div class=\"all_font\"><\/div>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div id=\"pgc-1949-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1949-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div><\/div>\n<p style=\"text-align: center;\"><strong style=\"color: #008000; text-align: center;\">DNA Sequence of Multiple Cloning Sites (MCS)<\/strong><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2880\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-cutting-sites.jpg\" alt=\"\" width=\"819\" height=\"722\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-cutting-sites.jpg 3857w, https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-cutting-sites-768x677.jpg 768w\" sizes=\"(max-width: 819px) 100vw, 819px\" \/><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14px;\">\u00a0<\/span><\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2877\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzyme-sites-of-TA-expression.jpg\" alt=\"\" width=\"879\" height=\"406\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzyme-sites-of-TA-expression.jpg 4193w, https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzyme-sites-of-TA-expression-768x355.jpg 768w\" sizes=\"(max-width: 879px) 100vw, 879px\" \/><\/p>\n<p style=\"text-align: center;\">\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2879\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-DO-NOT-cut-TA-exrpession.jpg\" alt=\"\" width=\"878\" height=\"628\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-DO-NOT-cut-TA-exrpession.jpg 4185w, https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-DO-NOT-cut-TA-exrpession-768x549.jpg 768w\" sizes=\"(max-width: 878px) 100vw, 878px\" \/><\/p>\n<p style=\"text-align: center;\">\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2878\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-cut-TA-expression.jpg\" alt=\"\" width=\"879\" height=\"1309\" \/><\/p>\n<p style=\"text-align: center;\">[su_divider]<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Support\/ Documents&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|700|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div id=\"pl-1384\" class=\"panel-layout\">\n<div id=\"pg-1384-0\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1949-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1949-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<table width=\"1679\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><span style=\"color: #000000;\"><strong>Manual<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/TA-expression-kit-new.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2535 alignnone\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Description The T&amp;A\u2122 Expression Kit (C-terminal) (F [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1949\"  class=\"panel-layout\" ><div id=\"pg-1949-0\"  class=\"panel-grid panel-no-style\"  data-style=\"{\"cell_alignment\":\"flex-start\"}\" ><div id=\"pgc-1949-0-0\"  class=\"panel-grid-cell\"  data-weight=\"0.61803399209206\" ><div id=\"panel-1949-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\t<div>\r\n<p><strong>Description<\/strong><\/p>\r\n<p>The key to protein expression is an expression vector, Yeastern Biotech\u2019s T&ATM Expression Kit (C-terminal) combine fast ligation of T-A cloning and expressing features of the T7 promoter. It offers a quick and efficient method of protein expression.<\/p>\r\n<p>The T&A\u2122 Expression Kit (C-terminal) (FYC201-10P) contains the T&A\u2122 Expression Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3\u2019-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS\u2122).<\/p>\r\n<\/div>\r\n<div class=\"all_font\">\r\n<div>\r\n<p><b>Features<\/b><\/p>\r\n<ul>\r\n<li>Fast ligation, completed in only 5 minutes<\/li>\r\n<li>Bacteriophage T7 promoter for high-level expression<\/li>\r\n<li>C-terminal 6x His tag for detection or purification<\/li>\r\n<li>High transformation efficiency<\/li>\r\n<li>Fast ligation, completed in only 5 minutes<\/li>\r\n<li>High transformation efficiency<\/li>\r\n<li>Two types of ligation buffers provided for your convenience<\/li>\r\n<li>Contain ampicillin marker for antibiotic selection<\/li>\r\n<li>Include T7 promoter primer sites for convenient sequencing<\/li>\r\n<\/ul>\r\n<p><b>Application<\/b><\/p>\r\n<ul>\r\n<li>Gene construction and protein expression.<\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<p>\u00a0<\/p>\r\n<p>\u00a0<\/p>\r\n<p>\u00a0<\/p>\r\n<p>\u00a0<\/p>\r\n<p><strong>Map & Sequence Reference Points<\/strong><\/p>\r\n<p><img class=\"alignnone size-full wp-image-2876\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-vector.jpg\" alt=\"\" width=\"3673\" height=\"4793\" \/><\/p>\r\n<p>\u00a0<\/p>\r\n<p>\u00a0<\/p>\r\n<p><strong>DNA Sequence of Multiple Cloning Sites (MCS)<\/strong><\/p>\r\n<p><img class=\"alignnone size-full wp-image-2880\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/TA-expression-cutting-sites.jpg\" alt=\"\" width=\"3857\" height=\"3401\" \/><\/p>\r\n<p><img class=\"alignnone size-full wp-image-2877\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzyme-sites-of-TA-expression.jpg\" alt=\"\" width=\"4193\" height=\"1937\" \/><\/p>\r\n<p><img class=\"alignnone size-full wp-image-2879\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-DO-NOT-cut-TA-exrpession.jpg\" alt=\"\" width=\"4185\" height=\"2993\" \/><\/p>\r\n<p><img class=\"alignnone size-full wp-image-2878\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Restriction-enzymes-that-cut-TA-expression.jpg\" alt=\"\" width=\"4025\" height=\"6001\" \/><\/p>\r\n<p>[su_divider]<\/p><\/div>\r\n<\/div><\/div><\/div><div id=\"pgc-1949-0-1\"  class=\"panel-grid-cell\"  data-weight=\"0.38196600790794\" ><div id=\"panel-1949-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n\t<p><strong>#FYC201-10P (10 preps)<\/strong><\/p>\r\n<p><strong>T&A<sup>TM<\/sup> Expression Kit\u00a0 (C-terminal) <\/strong><\/p>\r\n<table>\r\n<tbody>\r\n<tr>\r\n<td width=\"322\">T&A Expression Vector\u00a0 (C-terminal)<\/td>\r\n<td width=\"183\">20 \u03bcl (25 ng\/\u03bcl)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">Control Insert DNA (C-terminal)<\/td>\r\n<td width=\"183\">10 \u03bcl (10 ng\/\u03bcl)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">yT4 DNA Ligase (2U)<\/td>\r\n<td width=\"183\">10 \u03bcl<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">10X Ligation Buffer A<\/td>\r\n<td width=\"183\">50 \u03bcl<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">10X Ligation Buffer B<\/td>\r\n<td width=\"183\">50 \u03bcl<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">Primer T7 promoter<\/td>\r\n<td width=\"183\">50 \u03bcl (10\u03bcM)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"322\">Primer T7 terminator<\/td>\r\n<td width=\"183\">50 \u03bcl (10\u03bcM)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<p><strong>\u00a0<\/strong><\/p>\r\n<p><strong>#FYC211-10P (10 preps) <\/strong><\/p>\r\n<p><strong>T&A<sup>TM<\/sup> Expression Vector (C-terminal)<\/strong><\/p>\r\n<table>\r\n<tbody>\r\n<tr>\r\n<td width=\"346\">T&A Expression Vector\u00a0 (C-terminal)<\/td>\r\n<td width=\"151\">20 \u03bcl (25 ng\/\u03bcl)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"346\">Control Insert DNA (C-terminal)<\/td>\r\n<td width=\"151\">10 \u03bcl (10 ng\/\u03bcl)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"346\">Primer T7 promoter<\/td>\r\n<td width=\"151\">50 \u03bcl (10\u03bcM)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"346\">Primer T7 terminator<\/td>\r\n<td width=\"151\">50 \u03bcl (10\u03bcM)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<table style=\"height: 56px;\" width=\"1679\">\r\n<tbody>\r\n<tr>\r\n<td style=\"text-align: left;\">Manual<\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"text-align: left;\"><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/TA-expression-kit-new.pdf\">\u00a0\u00a0<img class=\"wp-image-2535 alignnone\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table><\/div>\r\n<\/div><\/div><\/div><\/div><\/div>","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1949"}],"collection":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1949"}],"version-history":[{"count":20,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1949\/revisions"}],"predecessor-version":[{"id":6891,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1949\/revisions\/6891"}],"wp:attachment":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1949"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}