Description<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\nRealStart DNA Polymerase premix is an ultra-sensitive and convenient PCR premix product. It contains 2\u00d7 concentrated solution of HotStart DNA polymerase, dNTPs, optimized buffers, and loading dye (optional) needed for PCR. The only step it takes to perform PCR with RealStart DNA Polymerase premix is to add DNA template and primers into the reaction mix. Since special HotStart DNA polymerase in the premix is activated after heating, it greatly reduces non-specific amplification when working with the premix at room temperature.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\n<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n- \n
\n- Simple & time-saving<\/strong>\u00a0\u2013
Just add a template of interest and primers into the RealStart DNA Polymerase Premix<\/li>\n- Less Contamination<\/strong>\u00a0\u2013
Reduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase<\/li>\n- High Sensitivity<\/strong>\u00a0\u2013
tested in amplification of a single gene copy<\/li>\n- Convenient\u00a0<\/strong>\u2013
an excellent tool when working with high quantities of samples.<\/li>\n- Sample Size<\/strong>\u00a0\u2013
work excellent for short DNA templates (size shorter than 600 bp). Amplification of long targets up to 10 kb from genomic DNA.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\nApplications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n\n- High throughput hot-start PCR.<\/li>\n
- RT-PCR.<\/li>\n
- Highly specific amplification of complex genomic and cDNA templates.<\/li>\n
- Amplification of low copy DNA targets.<\/li>\n
- Generation of PCR products for TA cloning.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\")
<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n# FYT101-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n2\u00d7 RealStart DNA Polymerase<\/td>\n 1.25 ml<\/td>\n<\/tr>\n \nPremix (w\/o loading dye)<\/td>\n 0.1U\/\u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\nPremix contains:\u00a0<\/b><\/span><\/p>\n\n\u25a0 Hotstart Taq DNA polymerase<\/div>\n\u25a0 dNTPs mix (including dATP,
dCTP, dGTP, dTTP)<\/div>\n\u25a0 7.5 mM MgCl2<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Technical” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n\n<\/div>\n<\/div>\n\n\nUnit Definition<\/strong><\/p>\n<\/div>\n<\/div>\n\n\nOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into the acid-insoluble material in 30 minutes at 72\u00b0C.<\/p>\n
<\/p>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n\n\n- Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of RealStart DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C<\/li>\n
- The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\nResults<\/strong><\/div>\n\n
![\"\"](\"http:\/\/www.yeastern.com\/updfile\/upload\/pcr1-5-1.png\")
\u00a0![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/RealStart-DNA-Polymerase-premix-data.jpg\")
<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[su_divider]<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
<\/strong><\/h6>\n<\/strong><\/h6>\n<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n- \n
\n- Simple & time-saving<\/strong>\u00a0\u2013
Just add a template of interest and primers into the RealStart DNA Polymerase Premix<\/li>\n- Less Contamination<\/strong>\u00a0\u2013
Reduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase<\/li>\n- High Sensitivity<\/strong>\u00a0\u2013
tested in amplification of a single gene copy<\/li>\n- Convenient\u00a0<\/strong>\u2013
an excellent tool when working with high quantities of samples.<\/li>\n- Sample Size<\/strong>\u00a0\u2013
work excellent for short DNA templates (size shorter than 600 bp). Amplification of long targets up to 10 kb from genomic DNA.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\nApplications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n\n- High throughput hot-start PCR.<\/li>\n
- RT-PCR.<\/li>\n
- Highly specific amplification of complex genomic and cDNA templates.<\/li>\n
- Amplification of low copy DNA targets.<\/li>\n
- Generation of PCR products for TA cloning.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n# FYT101-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n2\u00d7 RealStart DNA Polymerase<\/td>\n 1.25 ml<\/td>\n<\/tr>\n \nPremix (w\/o loading dye)<\/td>\n 0.1U\/\u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\nPremix contains:\u00a0<\/b><\/span><\/p>\n\n\u25a0 Hotstart Taq DNA polymerase<\/div>\n\u25a0 dNTPs mix (including dATP,
dCTP, dGTP, dTTP)<\/div>\n\u25a0 7.5 mM MgCl2<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Technical” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n\n<\/div>\n<\/div>\n\n\nUnit Definition<\/strong><\/p>\n<\/div>\n<\/div>\n\n\nOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into the acid-insoluble material in 30 minutes at 72\u00b0C.<\/p>\n
<\/p>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n\n\n- Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of RealStart DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C<\/li>\n
- The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\nResults<\/strong><\/div>\n\n
\u00a0<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[su_divider]<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n- \n
\n- Simple & time-saving<\/strong>\u00a0\u2013
Just add a template of interest and primers into the RealStart DNA Polymerase Premix<\/li>\n- Less Contamination<\/strong>\u00a0\u2013
Reduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase<\/li>\n- High Sensitivity<\/strong>\u00a0\u2013
tested in amplification of a single gene copy<\/li>\n- Convenient\u00a0<\/strong>\u2013
an excellent tool when working with high quantities of samples.<\/li>\n- Sample Size<\/strong>\u00a0\u2013
work excellent for short DNA templates (size shorter than 600 bp). Amplification of long targets up to 10 kb from genomic DNA.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\nApplications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n\n- High throughput hot-start PCR.<\/li>\n
- RT-PCR.<\/li>\n
- Highly specific amplification of complex genomic and cDNA templates.<\/li>\n
- Amplification of low copy DNA targets.<\/li>\n
- Generation of PCR products for TA cloning.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n# FYT101-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n2\u00d7 RealStart DNA Polymerase<\/td>\n 1.25 ml<\/td>\n<\/tr>\n \nPremix (w\/o loading dye)<\/td>\n 0.1U\/\u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\nPremix contains:\u00a0<\/b><\/span><\/p>\n\n\u25a0 Hotstart Taq DNA polymerase<\/div>\n\u25a0 dNTPs mix (including dATP,
dCTP, dGTP, dTTP)<\/div>\n\u25a0 7.5 mM MgCl2<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Technical” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n\n<\/div>\n<\/div>\n\n\nUnit Definition<\/strong><\/p>\n<\/div>\n<\/div>\n\n\nOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into the acid-insoluble material in 30 minutes at 72\u00b0C.<\/p>\n
<\/p>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n\n\n- Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of RealStart DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C<\/li>\n
- The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\nResults<\/strong><\/div>\n\n
\u00a0<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[su_divider]<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
- \n
- \n
- \n
- Simple & time-saving<\/strong>\u00a0\u2013
Just add a template of interest and primers into the RealStart DNA Polymerase Premix<\/li>\n- Less Contamination<\/strong>\u00a0\u2013
Reduce non-specific amplification caused by mispriming events that occur during setup and initial temperature increase<\/li>\n- High Sensitivity<\/strong>\u00a0\u2013
tested in amplification of a single gene copy<\/li>\n- Convenient\u00a0<\/strong>\u2013
an excellent tool when working with high quantities of samples.<\/li>\n- Sample Size<\/strong>\u00a0\u2013
work excellent for short DNA templates (size shorter than 600 bp). Amplification of long targets up to 10 kb from genomic DNA.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\nApplications<\/strong><\/h6>\n
\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n<\/strong><\/div>\n\n\n- \n
- High throughput hot-start PCR.<\/li>\n
- RT-PCR.<\/li>\n
- Highly specific amplification of complex genomic and cDNA templates.<\/li>\n
- Amplification of low copy DNA targets.<\/li>\n
- Generation of PCR products for TA cloning.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n
<\/p>\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n
<\/strong><\/span><\/h6>\n
<\/h6>\n<\/div>\n
\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n
<\/strong><\/h6>\n
Specifications<\/strong><\/h6>\n
# FYT101-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/h6>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n 2\u00d7 RealStart DNA Polymerase<\/td>\n 1.25 ml<\/td>\n<\/tr>\n \n Premix (w\/o loading dye)<\/td>\n 0.1U\/\u03bcl<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n Premix contains:\u00a0<\/b><\/span><\/p>\n
\n\u25a0 Hotstart Taq DNA polymerase<\/div>\n\u25a0 dNTPs mix (including dATP,
dCTP, dGTP, dTTP)<\/div>\n\u25a0 7.5 mM MgCl2<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[\/et_pb_toggle][et_pb_toggle title=”Technical” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
<\/div>\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n\n\n<\/div>\n<\/div>\n\n\nUnit Definition<\/strong><\/p>\n<\/div>\n<\/div>\n
\n\nOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into the acid-insoluble material in 30 minutes at 72\u00b0C.<\/p>\n
<\/p>\n<\/div>\n<\/div>\n
Quality Control<\/strong><\/div>\n\n\n- \n
- Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of RealStart DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C<\/li>\n
- The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Results<\/strong><\/div>\n\n\u00a0<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n[su_divider]<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Support\/ Documents” _builder_version=”4.16″ title_level=”h4″ title_font=”|700|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n\n\n\n\n<\/sub><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n - The absence of endo-, exodeoxyribonucleases and ribonucleases are confirmed by appropriate tests. A functional test is performed by PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
- Nuclease activity is not detected after incubation of 1 \u03bcg \u03bb \/Hind<\/em>\u00a0III DNA with 5 units of RealStart DNA polymerase in 50 \u03bcl reaction buffer for 18 hours at 37 \u00b0C<\/li>\n
- Less Contamination<\/strong>\u00a0\u2013
- Simple & time-saving<\/strong>\u00a0\u2013
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<\/a><\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n