Description<\/strong><\/h6>\n\n\n\n\n\nThe EZtime Real-Time PCR Premix (# FYT103-100P<\/strong>\u00a0or\u00a0# FYT104-100P<\/strong>) is a ready-to-use, 2\u00d7 concentrated PCR premix, including Hotstart Taq, SYBR Green I, optimized reaction buffer and nucleotides (# FYT104-100P\u00a0<\/strong>with ROX) for running quantitative real-time PCR, including qPCR and 2-step qRT-PCR, in the SYBR Green I detection format. SYBR Green Premix provides high sensitivity, wide dynamic range, and reproducibility for quantification.<\/p>\n\n\n\n\n\n\nThe EZtime Real-Time PCR Premix (# FYT105-100P<\/strong>\u00a0or\u00a0# FYT106-100P<\/strong>) is a ready-to-use, 2\u00d7 concentrated PCR premix that contains all reagents (except for primers, probe, and template) needed for running real-time PCR (# FYT106-100P<\/strong>\u00a0with ROX). Real-time PCR is performed by addition of various probes, such as TaqMan, Molecular Beacon, etc. This product combines the high-performance Hotstart Taq with a buffer which provides good specificity, amplification efficiency for real-time PCR.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\n<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/strong><\/div>\n\n\n\n- \n
\n- Specificity :\u00a0<\/strong>Hotstart Taq and the optimized buffer eliminate non-specific amplification and formation of primer dimers<\/li>\n
- Sensitivity :\u00a0<\/strong>detects low copy number targets<\/li>\n
- Wide linear range :\u00a0<\/strong>accurate quantification across 9 orders of magnitude<\/li>\n
- Reproducibility and convenience :\u00a0<\/strong>ready-to-use 2\u00d7 master mix minimizes pipetting error and reduces set-up time<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n
Applications<\/strong><\/h6>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/strong><\/div>\n\n\n\n- Quantitative real-time PCR<\/li>\n
- Quantitative 2-step RT-PCR<\/li>\n
- Quick and accurate detection and quantification of a target gene through real-time PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.0.7″ title_level=”h4″ title_font=”|600|||||||”]<\/p>\n
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The EZtime Real-Time PCR Premix (# FYT103-100P<\/strong>\u00a0or\u00a0# FYT104-100P<\/strong>) is a ready-to-use, 2\u00d7 concentrated PCR premix, including Hotstart Taq, SYBR Green I, optimized reaction buffer and nucleotides (# FYT104-100P\u00a0<\/strong>with ROX) for running quantitative real-time PCR, including qPCR and 2-step qRT-PCR, in the SYBR Green I detection format. SYBR Green Premix provides high sensitivity, wide dynamic range, and reproducibility for quantification.<\/p>\n The EZtime Real-Time PCR Premix (# FYT105-100P<\/strong>\u00a0or\u00a0# FYT106-100P<\/strong>) is a ready-to-use, 2\u00d7 concentrated PCR premix that contains all reagents (except for primers, probe, and template) needed for running real-time PCR (# FYT106-100P<\/strong>\u00a0with ROX). Real-time PCR is performed by addition of various probes, such as TaqMan, Molecular Beacon, etc. This product combines the high-performance Hotstart Taq with a buffer which provides good specificity, amplification efficiency for real-time PCR.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n <\/strong><\/p>\n [\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.0.7″ title_level=”h4″ title_font=”|600|||||||”]<\/p>\n<\/strong><\/h6>\n
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Store at<\/strong><\/span><\/h6>\n
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