Description<\/strong><\/h6>\n\n\nDeoxy+ HiSpec Reverse Transcriptase (RT) is genetically engineered by introducing of point mutations to MMLV RT that increase half-life, reduce RNase activity and increase thermal stability. These designed mutations lead to increased specificity of Deoxy+ HiSpec RT and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA samples. It synthesizes a complementary DNA strand from total RNA, mRNA, or an RNA: DNA hybrid.<\/p>\n<\/div>\n
Deoxy+ RT Kit is composed of Deoxy+ HiSpec Reverse Transcriptase (RTase), 2X Deoxy+ RT premix, RNase inhibitor, and primers.<\/p>\n<\/div>\n
<\/strong><\/h6>\n<\/strong><\/h6>\n<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n- Half-life of 100 minutes at 50\u00b0C for the highest cDNA yields<\/li>\n
- Reduced RNase H activity for more full-length cDNA<\/li>\n
- Full activity at 50\u00b0C for increased specificity with GSP<\/li>\n
- Ability to increase RT units without inhibiting subsequent PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Applications<\/strong><\/h6>\n\n\n\n- Synthesis of the first-strand cDNA<\/li>\n
- Array labeling<\/li>\n
- cDNA libraries<\/li>\n
- RT-PCR, primer extension, and 3′ and 5′ RACE<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\")
<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\u00a0# FYT501-50R<\/b>\u00a0(50 rxns)<\/p>\n\n\n\n\n\n\n
Deoxy+ HiSpec Reverse Transcriptase (RT) is genetically engineered by introducing of point mutations to MMLV RT that increase half-life, reduce RNase activity and increase thermal stability. These designed mutations lead to increased specificity of Deoxy+ HiSpec RT and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA samples. It synthesizes a complementary DNA strand from total RNA, mRNA, or an RNA: DNA hybrid.<\/p>\n<\/div>\n
Deoxy+ RT Kit is composed of Deoxy+ HiSpec Reverse Transcriptase (RTase), 2X Deoxy+ RT premix, RNase inhibitor, and primers.<\/p>\n<\/div>\n
<\/strong><\/h6>\n<\/strong><\/h6>\n<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n- Half-life of 100 minutes at 50\u00b0C for the highest cDNA yields<\/li>\n
- Reduced RNase H activity for more full-length cDNA<\/li>\n
- Full activity at 50\u00b0C for increased specificity with GSP<\/li>\n
- Ability to increase RT units without inhibiting subsequent PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Applications<\/strong><\/h6>\n\n\n\n- Synthesis of the first-strand cDNA<\/li>\n
- Array labeling<\/li>\n
- cDNA libraries<\/li>\n
- RT-PCR, primer extension, and 3′ and 5′ RACE<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\u00a0# FYT501-50R<\/b>\u00a0(50 rxns)<\/p>\n\n\n\n\n\n\n
<\/strong><\/h6>\nFeatures<\/strong><\/h6>\n\n\n\n- Half-life of 100 minutes at 50\u00b0C for the highest cDNA yields<\/li>\n
- Reduced RNase H activity for more full-length cDNA<\/li>\n
- Full activity at 50\u00b0C for increased specificity with GSP<\/li>\n
- Ability to increase RT units without inhibiting subsequent PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Applications<\/strong><\/h6>\n\n\n\n- Synthesis of the first-strand cDNA<\/li>\n
- Array labeling<\/li>\n
- cDNA libraries<\/li>\n
- RT-PCR, primer extension, and 3′ and 5′ RACE<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n<\/p>\n
\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n<\/strong><\/span><\/h6>\n<\/h6>\n<\/div>\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n<\/strong><\/h6>\nSpecifications<\/strong><\/h6>\n\n\n\n\n\n\n\n\n\n\n\n\n\u00a0# FYT501-50R<\/b>\u00a0(50 rxns)<\/p>\n\n\n\n\n\n\n
- \n
- Half-life of 100 minutes at 50\u00b0C for the highest cDNA yields<\/li>\n
- Reduced RNase H activity for more full-length cDNA<\/li>\n
- Full activity at 50\u00b0C for increased specificity with GSP<\/li>\n
- Ability to increase RT units without inhibiting subsequent PCR<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
Applications<\/strong><\/h6>\n
\n\n- \n
- Synthesis of the first-strand cDNA<\/li>\n
- Array labeling<\/li>\n
- cDNA libraries<\/li>\n
- RT-PCR, primer extension, and 3′ and 5′ RACE<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
[\/et_pb_toggle][et_pb_toggle title=”Specifications” _builder_version=”4.16″ title_level=”h4″ title_font=”|600|||||||” global_colors_info=”{}”]<\/p>\n
\n\n\nStore at<\/strong><\/span><\/h6>\n
<\/p>\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/h6>\n
<\/strong><\/span><\/h6>\n
<\/h6>\n<\/div>\n
\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n
<\/strong><\/h6>\n
Specifications<\/strong><\/h6>\n
\n\n\n\n\n\n\n\n\n\n\n\n\u00a0# FYT501-50R<\/b>\u00a0(50 rxns)<\/p>\n
\n\n\n\n\n\n
![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\")
<\/a><\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Deoxy-HiSpec-Reverse-Transcriptase-data.png\"){kind=spacer}
<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n![\"\"](\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/Deoxy-HiSpec-Reverse-Transcriptase-data.png\"){kind=spacer}
<\/div>\r\n![\"\"](\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\")
<\/p>\r\n