{"id":1413,"date":"2017-07-11T14:13:51","date_gmt":"2017-07-11T06:13:51","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1413"},"modified":"2024-05-31T16:51:59","modified_gmt":"2024-05-31T08:51:59","slug":"deoxy-real-time-sybr-green-rt-pcr-kit","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1413","title":{"rendered":"Deoxy+ Real-time SYBR Green RT-PCR Kit"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;section&#8221; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_4,3_4&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; width=&#8221;85%&#8221; max_width=&#8221;2560px&#8221; module_alignment=&#8221;center&#8221; custom_padding=&#8221;30px|0px|0px|0px|false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_menu menu_id=&#8221;76&#8243; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.25.1&#8243; menu_font=&#8221;|600|||||||&#8221; menu_text_color=&#8221;#474747&#8243; menu_font_size=&#8221;15px&#8221; width=&#8221;76%&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; menu_text_shadow_style=&#8221;preset1&#8243; sticky_enabled=&#8221;0&#8243;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; _builder_version=&#8221;4.25.1&#8243; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; title_font=&#8221;|600|||||||&#8221; sticky_enabled=&#8221;0&#8243;][\/et_pb_post_title][et_pb_text _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_text][et_pb_toggle title=&#8221;Product overview&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|800|||||||&#8221; closed_title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div id=\"pgc-1413-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1413-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6 class=\"all_font\"><strong><span>Description<\/span><\/strong><\/h6>\n<\/div>\n<div>\n<div>\n<div>\n<p>Deoxy+ Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. Yeastern\u2019s Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer\u2013dimers.<\/p>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Features<\/strong><\/h6>\n<div>\n<div>\n<ul>\n<li>Ready-to-use master mix to allow faster setup, maximize throughput and reduce the contamination risk of real-time RT-PCR analysis<\/li>\n<li>Detection of even low copy numbers<\/li>\n<li>Excellent sensitivity and easy optimization with SYBR Green<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Applications<\/strong><\/h6>\n<\/div>\n<\/div>\n<\/div>\n<ul>\n<li>Validation of siRNA-mediated gene knockdown<\/li>\n<li>Detection of gene regulation<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Specifications&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><span style=\"color: #000000;\"><strong>Store at<\/strong><\/span><\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2357\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\" alt=\"\" width=\"114\" height=\"84\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-230x170.png 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C.png 327w\" sizes=\"(max-width: 114px) 100vw, 114px\" \/><\/p>\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><\/h6>\n<\/div>\n<div>\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div id=\"pgc-1384-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1384-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Specifications<\/strong><\/h6>\n<div>\n<div id=\"pgc-1384-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1384-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div id=\"pgc-1949-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1949-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p>\u00a0<b># FYT504-50P<\/b>\u00a0(50 preps)\u00a0<\/p>\n<div id=\"pgc-1413-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1413-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div>\n<table border=\"0\" width=\"210\">\n<tbody>\n<tr>\n<td>2\u00d7 Deoxy+ Real-time<br \/> SYBR Green RT PCR Premix, ROX<\/td>\n<td align=\"right\" width=\"100\">0.625ml<\/td>\n<\/tr>\n<tr>\n<td>Sterilized ddH<sub>2<\/sub>O<\/td>\n<td align=\"right\">1 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYT504-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/p>\n<div>\n<table border=\"0\" width=\"210\">\n<tbody>\n<tr>\n<td>2\u00d7 Deoxy+ Real-time<br \/> SYBR Green RT PCR Premix, ROX<\/td>\n<td align=\"right\" width=\"100\">0.625ml\u00d72<\/td>\n<\/tr>\n<tr>\n<td>Sterilized ddH<sub>2<\/sub>O<\/td>\n<td align=\"right\">1 ml\u00d7 2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Technical&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div id=\"pgc-1413-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1413-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p><strong>Quality Control<\/strong><\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div>\n<p>The performance of Deoxy+ Real-time SYBR Green RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)18. The sensitivity of the kit is verified by the detection of B2M transcript after 35 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Support\/ Documents&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|700|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div id=\"pgc-1413-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1413-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1413-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1413-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"all_font\" style=\"text-align: center;\">\n<div><\/div>\n<\/div>\n<table width=\"1679\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><strong>Manual<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/Deoxy_Real-Time-SYBR-Green-RT-PCR-Kit-Reverse-Transcription.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2535\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Description Deoxy+ Real-time SYBR Green RT-PCR system p [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1413\"  class=\"panel-layout\" ><div id=\"pg-1413-0\"  class=\"panel-grid panel-no-style\"  data-style=\"{&quot;cell_alignment&quot;:&quot;flex-start&quot;}\" ><div id=\"pgc-1413-0-0\"  class=\"panel-grid-cell\"  data-weight=\"0.61803399209206\" ><div id=\"panel-1413-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div class=\"all_font\"><strong><span style=\"font-size: 14px; letter-spacing: 0px;\">Description<\/span><\/strong><\/div>\n<\/div>\n<div>\n<div>\n<div>\n<p>Deoxy+ Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. Yeastern\u2019s Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer\u2013dimers.<\/p>\n<\/div>\n<\/div>\n<div><strong>Features<\/strong><\/div>\n<div>\n<div>\n<ul>\n<li>Ready-to-use master mix to allow faster setup, maximize throughput and reduce the contamination risk of real-time RT-PCR analysis<\/li>\n<li>Detection of even low copy numbers<\/li>\n<li>Excellent sensitivity and easy optimization with SYBR Green<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div><strong>Applications<\/strong><\/div>\n<\/div>\n<\/div>\n<\/div>\n<ul>\n<li>Validation of siRNA-mediated gene knockdown<\/li>\n<li>Detection of gene regulation<\/li>\n<\/ul>\n<div><strong style=\"font-size: 14px; letter-spacing: 0px;\">Quality Control<\/strong><\/div>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div>\n<p>The performance of Deoxy+ Real-time SYBR Green RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)18. The sensitivity of the kit is verified by the detection of B2M transcript after 35 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"all_font\">\u00a0<\/div><\/div>\n<\/div><\/div><\/div><div id=\"pgc-1413-0-1\"  class=\"panel-grid-cell\"  data-weight=\"0.38196600790794\" ><div id=\"panel-1413-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"all_font\">Specification<\/div>\n<div class=\"all_font\">\n<p><span style=\"color: #333333;\">Store at<\/span><\/p>\n<p><img class=\"alignnone wp-image-2357\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/20C-300x222.png\" alt=\"\" width=\"126\" height=\"93\" \/><\/p>\n<p><span style=\"color: #333333;\"><b># FYT504-50P<\/b>\u00a0(50 preps)\u00a0<\/span><\/p>\n<div>\n<table border=\"0\" width=\"210\">\n<tbody>\n<tr>\n<td>2\u00d7 Deoxy+ Real-time<br \/> SYBR Green RT PCR Premix, ROX<\/td>\n<td align=\"right\" width=\"100\">0.625 ml<\/td>\n<\/tr>\n<tr>\n<td>Sterilized ddH<sub>2<\/sub>O<\/td>\n<td align=\"right\">1 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span style=\"color: #333333;\"><b># FYT504-100P<\/b>\u00a0(100 preps)\u00a0<\/span><\/p>\n<div>\n<table border=\"0\" width=\"210\">\n<tbody>\n<tr>\n<td>2\u00d7 Deoxy+ Real-time<br \/> SYBR Green RT PCR Premix, ROX<\/td>\n<td align=\"right\" width=\"100\">0.625 ml\u00d7 2<\/td>\n<\/tr>\n<tr>\n<td>Sterilized ddH<sub>2<\/sub>O<\/td>\n<td align=\"right\">1 ml\u00d7 2<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<table style=\"height: 56px;\" width=\"1679\">\n<tbody>\n<tr>\n<td style=\"text-align: left;\">Manual<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: left;\"><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/09\/FYO001-100G-Glass-Plating-Beads-Sterilized-Manual.pdf\">\u00a0\u00a0<\/a><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/Deoxy_Real-Time-SYBR-Green-RT-PCR-Kit-Reverse-Transcription.pdf\"><img class=\"alignnone wp-image-2535\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/div><\/div><\/div><\/div><\/div>","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1413"}],"collection":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1413"}],"version-history":[{"count":17,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1413\/revisions"}],"predecessor-version":[{"id":6898,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1413\/revisions\/6898"}],"wp:attachment":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1413"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}