![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/pYLEX1.jpg\")
![\"\"](\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/pYLSC1.jpg\")
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* INRA (Institut National de la Recherche Agronomique) and INAPG (Institut National Agronomique Paris-Grignon)<\/em><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/strong><\/h6>\n
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Features<\/strong><\/h6>\n
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<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\nApplications<\/strong><\/h6>\n
\n\n\n\n\n\n\n\n\n\n\n\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n\n\n<\/strong><\/div>\n\n\nHeterologous protein expression, either intracellular or extracellular depends on selected vector in GRAS yeast.<\/div>\n<\/div>\n<\/div>\nYarrowia Vectors<\/div>\n\n\nTwo vectors (pYLEX1 and pYLSC1) are included in this kit, and they can be used for either intracellular expression or secretion of proteins of interest in\u00a0Y. lipolytica.<\/em>\u00a0Generally speaking, if the target protein is cytosolic and non-glycosylated, the pYLEX1 vector is a better choice. If the protein of your interest is normally glycosylated or secreted, you may want to choose the pYLSC1 vector.<\/div>\n![\"\"](\"http:\/\/www.yeastern.com\/img\/upload\/image\/pYLEX1_correct.png\")
<\/div>\nThe pYLEX1 expression vector (7259 bp) contains the strong hybrid promoter (hp4d) carrying four tandem copies of upstream activator sequences (UAS1B) fragment from pXPR2 and a minimal pLEU2 fragment. The multiple cloning sites and the XPR2 transcription terminator lie immediately downstream of 3′ site of hp4d promoter, followed by a leucine selection marker gene (LEU2). The vector can be linearized by digestion with NotI to create a linear DNA fragment capable of inserting into the Y. lipolytica genome.<\/div>\n![\"\"](\"http:\/\/www.yeastern.com\/img\/upload\/image\/pYLSC1_correct.png\")
<\/div>\nThe pYLSC1 secretion vector (7205 bp) contains the hybrid promoter (hp4d) and a secretion signal (XPR2 pre region). The multiple cloning site and the pXPR2 transcription terminator lie immediately downstream of 3′ site of XPR2 pre region, followed by a leucine selection marker gene (LEU2). The vector can be linearized by digestion with NotI to create a linear DNA fragment capable of inserting into the Y. lipolytica genome.<\/div>\n<\/div>\n<\/div>\nYeast Strain<\/div>\n\nThe strain Po1g of Yarrowia lipolytica is a derivative of the wild-type strain W29 (ATCC 20460) by a series of genetic modifications. Briefly, the original URA3 gene in the W29 strain was disrupted with the SUC2 gene from Saccharomyces cerevisiae, followed by the introduction of a deletion in the LEU2 gene. Furthermore, the deletion of the XPR2 and AXP genes ensures that Po1g is unable to produce any extracellular protease. In order to allow easy integration of pBR-based expression\/secretion vectors, a pBR322 docking platform was integrated at the URA3 locus.<\/div>\n<\/div>\n<\/strong><\/h6>\n
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Related Product<\/strong><\/h6>\n
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