{"id":1384,"date":"2017-07-11T14:00:41","date_gmt":"2017-07-11T06:00:41","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1384"},"modified":"2024-05-31T16:37:13","modified_gmt":"2024-05-31T08:37:13","slug":"ta-cloning-kit","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1384","title":{"rendered":"T&A\u2122 Cloning Kit"},"content":{"rendered":"

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\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Multiple Cloning region<\/td>\n434 to 490<\/td>\n<\/tr>\n
LacZ start codon<\/td>\n165<\/td>\n<\/tr>\n
LacZ operator<\/td>\n185 to 201<\/td>\n<\/tr>\n
Lac Z gene<\/td>\n511 to 149<\/td>\n<\/tr>\n
Ori<\/td>\n909-1497<\/td>\n<\/tr>\n
Ampr gene<\/td>\n2528 to 1671<\/td>\n<\/tr>\n
T7 promoter<\/td>\n402 to 419<\/td>\n<\/tr>\n
M13 forward primer<\/td>\n359 to 375<\/td>\n<\/tr>\n
M13 reverse primer<\/td>\n507 to 528<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A\u2122\u00a0Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.<\/p>\n

The T&A\u2122\u00a0Cloning Kit contains the T&A\u2122\u00a0Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS\u2122) or be purified first to achieve higher transformation efficiency.<\/p>\n<\/div>\n<\/div>\n

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Features<\/strong><\/h6>\n
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