{"id":1378,"date":"2017-07-11T13:59:51","date_gmt":"2017-07-11T05:59:51","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1378"},"modified":"2024-05-31T16:33:15","modified_gmt":"2024-05-31T08:33:15","slug":"ecos-10b-competent-cells-dh10b","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1378","title":{"rendered":"ECOS\u2122 10B Competent Cells [DH10B]"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; admin_label=&#8221;section&#8221; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_4,3_4&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; width=&#8221;85%&#8221; max_width=&#8221;2560px&#8221; module_alignment=&#8221;center&#8221; custom_padding=&#8221;30px|0px|0px|0px|false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_menu menu_id=&#8221;76&#8243; active_link_color=&#8221;#000000&#8243; _builder_version=&#8221;4.25.1&#8243; menu_font=&#8221;|700|||||||&#8221; menu_text_color=&#8221;#474747&#8243; menu_font_size=&#8221;15px&#8221; width=&#8221;76%&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; menu_text_shadow_style=&#8221;preset1&#8243; sticky_enabled=&#8221;0&#8243;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; _builder_version=&#8221;4.25.1&#8243; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; title_font=&#8221;|600|||||||&#8221; sticky_enabled=&#8221;0&#8243;][\/et_pb_post_title][et_pb_text _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2020\/02\/10b.png\" width=\"707\" height=\"555\" alt=\"\" class=\"wp-image-4016 alignnone size-full\" \/><\/p>\n<p>[\/et_pb_text][et_pb_toggle title=&#8221;Product overview&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|800|||||||&#8221; closed_title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><strong>Description<\/strong><\/h6>\n<\/div>\n<div>\n<div id=\"pgc-1378-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div>\n<p>DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.<\/p>\n<p>While DH10B has been classically reported to be\u00a0<em>gal<\/em>U\u00a0<em>gal<\/em>K, the preliminary genome sequence for DH10B indicates that DH10B is actually\u00a0<em>gal<\/em>E\u00a0<em>gal<\/em>K\u00a0<em>gal<\/em>U+. Genome sequence indicates that DH10B is actually\u00a0<em>deo<\/em>R+. The complete genome sequence has been published.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1378-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Features<\/strong><\/h6>\n<div id=\"pgc-1378-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div><\/div>\n<div>\n<div>\n<ul>\n<li>Streptomycin resistant<\/li>\n<li>High transformation efficiency<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Applications<\/strong><\/h6>\n<div id=\"pgc-1378-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div><\/div>\n<\/div>\n<div><\/div>\n<div>\n<div>\n<ul>\n<li>Suitable for genomic DNA cloning<\/li>\n<li>Allow large inserts<\/li>\n<li>Cloning of methylated cytosine or adenine containing DNA<\/li>\n<li>Blue\/white screening<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1378-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<table width=\"1687\">\n<tbody>\n<tr>\n<td><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/ECOS-CARD-FULL.pdf\"><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Technical \/ Specifications&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><span style=\"color: #000000;\"><strong>Store at<\/strong><\/span><\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2329\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png\" alt=\"\" width=\"97\" height=\"71\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-230x170.png 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC.png 327w\" sizes=\"(max-width: 97px) 100vw, 97px\" \/><\/p>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong>Genotype<\/strong><\/span><\/h6>\n<\/div>\n<div>\n<div>\n<div id=\"pgc-1378-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div><\/div>\n<\/div>\n<div><\/div>\n<div>\n<div>F<sup>&#8211;<\/sup><em>end<\/em>A1<em>\u00a0rec<\/em>A1\u00a0<em>gal<\/em>E15\u00a0<em>gal<\/em>K16<em>\u00a0nup<\/em>G<em>\u00a0rps<\/em>L \u0394<em>lac<\/em>X74 \u03a680<em>lac<\/em>Z\u0394M15\u00a0<em>ara<\/em>D139 \u0394(ara,leu)7697\u00a0<em>mcr<\/em>A \u0394(mrr-hsdRMS-mcrBC) \u03bb<sup>&#8211;<\/sup><\/div>\n<\/div>\n<div><\/div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1378-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Specifications<\/strong><\/h6>\n<\/div>\n<\/div>\n<div class=\"all_font\">\n<div id=\"pgc-1378-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<p><span><b># FYE507-10VL<\/b>\u00a0(10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">10 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE507-80VL<\/b>\u00a0(80 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">80 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE508-10VL<\/b>\u00a0(10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">10 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE508-80VL<\/b>\u00a0(80 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">80 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE507-96WL<\/b>\u00a0(96 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>50 \u03bcl\/well<\/td>\n<td align=\"right\">96 wells<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Support\/ Documents&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|700|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div id=\"pgc-1378-0-0\" class=\"panel-grid-cell\" data-weight=\"0.61803399209206\">\n<div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pgc-1378-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\n<div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<div class=\"all_font\">\n<div><\/div>\n<\/div>\n<table width=\"1687\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><strong>Manual<\/strong><\/td>\n<td style=\"text-align: center;\"><strong>ECOS Card<\/strong><\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2023\/04\/ECOS-Manual-VerU0923-Reading-\u5df2\u58d3\u7e2e.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2535 aligncenter\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<td><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/ECOS-CARD-FULL.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2535 aligncenter\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Description DH10B is an MC1061 derivative. This strain  [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1378\"  class=\"panel-layout\" ><div id=\"pg-1378-0\"  class=\"panel-grid panel-no-style\"  data-style=\"{&quot;cell_alignment&quot;:&quot;flex-start&quot;}\" ><div id=\"pgc-1378-0-0\"  class=\"panel-grid-cell\"  data-weight=\"0.61803399209206\" ><div id=\"panel-1378-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<div class=\"all_font\"><b style=\"font-family: inherit; font-size: 16px; font-style: inherit; letter-spacing: 0px;\">Strain: DH10B<\/b><\/div>\n<div>\u00a0<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>Description<\/div>\n<div>\n<div>\n<p>DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.<\/p>\n<p>While DH10B has been classically reported to be\u00a0<em>gal<\/em>U\u00a0<em>gal<\/em>K, the preliminary genome sequence for DH10B indicates that DH10B is actually\u00a0<em>gal<\/em>E\u00a0<em>gal<\/em>K\u00a0<em>gal<\/em>U+. Genome sequence indicates that DH10B is actually\u00a0<em>deo<\/em>R+. The complete genome sequence has been published.<\/p>\n<\/div>\n<\/div>\n<div>Genotype<\/div>\n<div>\n<div>F<sup>-<\/sup><em>end<\/em>A1<em>\u00a0rec<\/em>A1\u00a0<em>gal<\/em>E15\u00a0<em>gal<\/em>K16<em>\u00a0nup<\/em>G<em>\u00a0rps<\/em>L \u0394<em>lac<\/em>X74 \u03a680<em>lac<\/em>Z\u0394M15\u00a0<em>ara<\/em>D139 \u0394(ara,leu)7697\u00a0<em>mcr<\/em>A \u0394(mrr-hsdRMS-mcrBC) \u03bb<sup>-<\/sup><\/div>\n<\/div>\n<div>\u00a0<\/div>\n<div>\u00a0<\/div>\n<div>Features<\/div>\n<div>\n<div>\n<ul>\n<li>Streptomycin resistant<\/li>\n<li>Prevent DNA methylation<\/li>\n<li>High transformation efficiency<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div>Applications<\/div>\n<div>\n<div>\n<ul>\n<li>Suitable for genomic DNA cloning<\/li>\n<li>Allow large inserts<\/li>\n<li>Cloning of methylated cytosine or adenine containing DNA<\/li>\n<li>Blue\/white screening<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div>Efficiency<\/div>\n<div>\n<div>\n<p><strong>&gt;5 x 10<sup>7<\/sup>\u00a0cfu\/ \u03bcg<\/strong><br \/>Recommended for :<\/p>\n<ul>\n<li>Subcloning<\/li>\n<li>Genomic DNA cloning<\/li>\n<\/ul>\n<p><strong>&gt;1 x 10<sup>8<\/sup>\u00a0cfu\/ \u03bcg<\/strong><br \/>Recommended for :<\/p>\n<ul>\n<li>Cloning of large insert plasmids<\/li>\n<li>Genomic DNA cloning<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"all_font\">\u00a0<\/div><\/div>\n<\/div><\/div><\/div><div id=\"pgc-1378-0-1\"  class=\"panel-grid-cell\"  data-weight=\"0.38196600790794\" ><div id=\"panel-1378-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_display&quot;:&quot;tile&quot;}\" ><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<div class=\"all_font\">Specification<\/div>\n<div class=\"all_font\">\n\n<span style=\"color: #333333;\">Store at<\/span>\n\n<img class=\"alignnone wp-image-2329\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png\" alt=\"\" width=\"106\" height=\"79\" \/>\n\n<span style=\"color: #333333;\"><b># FYE507-10VL<\/b>\u00a0(10 preps)<\/span>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">10 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<span style=\"color: #333333;\"><b># FYE507-80VL<\/b>\u00a0(80 preps)<\/span>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">80 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<span style=\"color: #333333;\"><b># FYE508-10VL<\/b>\u00a0(10 preps)<\/span>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">10 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<span style=\"color: #333333;\"><b># FYE508-80VL<\/b>\u00a0(80 preps)<\/span>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"90\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"90\">80 vials<\/td>\n<\/tr>\n<tr>\n<td>Control Plasmid (pUC19)<\/td>\n<td align=\"right\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<span style=\"color: #333333;\"><b># FYE507-96WL<\/b>\u00a0(96 preps)<\/span>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>7<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>50 \u03bcl\/well<\/td>\n<td align=\"right\">96 wells<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<table style=\"height: 55px;\" width=\"1687\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\">Manual<\/td>\n<td style=\"text-align: center;\">ECOS Card<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\"><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/ECOS-1-Minute-Transformation-Competent-Cells-DH5a-JM109-XL1-blue-10B-BL21-1.pdf\"><img class=\"wp-image-2535 aligncenter\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\n<td><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/ECOS-CARD-FULL.pdf\"><img class=\"wp-image-2535 aligncenter\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1378"}],"collection":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1378"}],"version-history":[{"count":23,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1378\/revisions"}],"predecessor-version":[{"id":6886,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1378\/revisions\/6886"}],"wp:attachment":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}