{"id":1376,"date":"2017-07-11T13:59:29","date_gmt":"2017-07-11T05:59:29","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1376"},"modified":"2024-05-31T16:31:36","modified_gmt":"2024-05-31T08:31:36","slug":"ecos-21-competent-cells-bl21de3","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1376","title":{"rendered":"ECOS\u2122 21 Competent Cells [BL21(DE3)]"},"content":{"rendered":"

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Description<\/strong><\/h6>\n<\/div>\n
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This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the\u00a0lac<\/em>UV5 promoter by isopropyl-1-thio-\u00df-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute\/ heat shock cold plating protocol is recommended if high efficiency is desired.<\/div>\n
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Genotype<\/div>\n
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F–<\/sup>\u00a0omp<\/em>T\u00a0gal dcm<\/em>\u00a0lon\u00a0hsd<\/em>SB<\/sub>(rB<\/sub>–<\/sup>\u00a0mB<\/sub>–<\/sup>) \u03bb(DE3 [lac<\/em>I\u00a0lac<\/em>UV5-T7 gene 1\u00a0ind<\/em>1\u00a0sam<\/em>7\u00a0nin<\/em>5])<\/div>\n<\/div>\n
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Features<\/strong><\/h6>\n
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