{"id":1374,"date":"2017-07-11T13:58:56","date_gmt":"2017-07-11T05:58:56","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1374"},"modified":"2024-05-31T16:34:31","modified_gmt":"2024-05-31T08:34:31","slug":"ecos-blue-competent-cells-xl1-blue","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1374","title":{"rendered":"ECOS\u2122 Blue Competent Cells [XL1-Blue]"},"content":{"rendered":"

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XL1-Blue is the most popular strain for blue\/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (end<\/em>A) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (rec<\/em>A) deficient, improving insert stability. The\u00a0hsd<\/em>R mutation prevents the cleavage of cloned DNA by the\u00a0Eco<\/em>K endonuclease system. The\u00a0lacI<\/em>qZ\u0394M15 gene on the F\u00b4 episome allows blue-white color screening.<\/div>\n<\/div>\n
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Features<\/strong><\/h6>\n
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