{"id":1372,"date":"2017-07-11T13:58:38","date_gmt":"2017-07-11T05:58:38","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1372"},"modified":"2024-05-31T16:28:14","modified_gmt":"2024-05-31T08:28:14","slug":"ecos-9-5-competent-cells-jm109","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1372","title":{"rendered":"ECOS\u2122 9-5 Competent Cells [JM109]"},"content":{"rendered":"

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JM109, a K strain bacterium, carries mutations in\u00a0rec<\/em>A and\u00a0end<\/em>A, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F\u2019 episome which allows blue\/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.<\/p>\n

This product can become a powerful cloning tool when it is used in combination with Yeastern\u2019s rapid ligation kit\u00a0(#FYC003-100R)<\/strong>. The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.<\/p>\n<\/div>\n<\/div>\n

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Features<\/strong><\/h6>\n
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