{"id":1370,"date":"2017-07-11T13:58:24","date_gmt":"2017-07-11T05:58:24","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1370"},"modified":"2024-05-31T16:22:20","modified_gmt":"2024-05-31T08:22:20","slug":"ecos-101-competent-cells-dh5a","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1370","title":{"rendered":"ECOS\u2122 101 Competent Cells [DH5\u03b1]"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;0px|0px|0px|0px|false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_4,3_4&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; width=&#8221;85%&#8221; max_width=&#8221;2560px&#8221; module_alignment=&#8221;center&#8221; custom_padding=&#8221;30px|0px|0px|0px|false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_menu menu_id=&#8221;76&#8243; active_link_color=&#8221;RGBA(255,255,255,0)&#8221; _builder_version=&#8221;4.25.1&#8243; menu_font=&#8221;|700|||||||&#8221; menu_text_color=&#8221;#575757&#8243; menu_font_size=&#8221;15px&#8221; background_enable_color=&#8221;off&#8221; width=&#8221;76%&#8221; custom_margin=&#8221;||||false|false&#8221; custom_margin_tablet=&#8221;&#8221; custom_margin_phone=&#8221;&#8221; custom_margin_last_edited=&#8221;on|desktop&#8221; custom_padding=&#8221;|32px||||&#8221; menu_text_shadow_style=&#8221;preset1&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_menu][\/et_pb_column][et_pb_column type=&#8221;3_4&#8243; _builder_version=&#8221;4.16&#8243; global_colors_info=&#8221;{}&#8221;][et_pb_post_title meta=&#8221;off&#8221; featured_image=&#8221;off&#8221; admin_label=&#8221;Post Title&#8221; _builder_version=&#8221;4.25.1&#8243; title_font=&#8221;|600|||||||&#8221; custom_margin=&#8221;||26px|||&#8221; hover_enabled=&#8221;0&#8243; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;][\/et_pb_post_title][et_pb_text _builder_version=&#8221;4.16&#8243; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2020\/02\/ECOS-.png\" width=\"452\" height=\"399\" alt=\"\" class=\"wp-image-3884 alignnone size-full\" \/>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2020\/02\/FYE608-80VL.png\" width=\"436\" height=\"358\" alt=\"\" class=\"wp-image-3888 alignnone size-full\" \/><\/p>\n<p>[\/et_pb_text][et_pb_toggle title=&#8221;Product overview&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|800|||||||&#8221; closed_title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><strong>Description<\/strong><\/h6>\n<\/div>\n<div>\n<p>DH5\u03b1 is the most popular <em>E. coli<\/em> strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries <em>rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries <em>end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/p>\n<\/div>\n<div><\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Features<\/strong><\/h6>\n<p>Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.<\/p>\n<div><\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Applications<\/strong><\/h6>\n<div>\n<div>\n<ul>\n<li>Cloning and subcloning<\/li>\n<li>Scale-up application<\/li>\n<li>Blue\/white screening<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Technical \/ Specifications&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|600|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div class=\"all_font\">\n<div class=\"all_font\">\n<div>\n<h6><span style=\"color: #000000;\"><strong>Store at<\/strong><\/span><\/h6>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-2329\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png\" alt=\"\" width=\"97\" height=\"71\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-230x170.png 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC.png 327w\" sizes=\"(max-width: 97px) 100vw, 97px\" \/><\/p>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong><\/strong><\/span><\/h6>\n<h6><span style=\"color: #000000;\"><strong>Genotype<\/strong><\/span><\/h6>\n<\/div>\n<div>\n<div><em>end<\/em>A1\u00a0<em>rec<\/em>A1\u00a0<em>rel<\/em>A1\u00a0<i>deoR\u00a0<\/i><em>gyr<\/em>A96 <em>nupG<\/em>\u00a0<em>hsd<\/em>R17(rk<sup>&#8211;<\/sup>, mk<sup>+<\/sup>)\u00a0<em>sup<\/em>E44<em>\u00a0thi<\/em>-1 \u0394(<em>lac<\/em>ZYA-<em>arg<\/em>F)U169 \u03a680 <em>lacZ<\/em>\u0394M15 <span>\u03bb<sup>&#8211;<\/sup><\/span><sup>\u00a0<\/sup>F<sup>&#8211;<\/sup><\/div>\n<div><\/div>\n<\/div>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong><\/strong><\/h6>\n<h6><strong>Specifications<\/strong><\/h6>\n<div>\n<div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"all_font\">\n<p><span><b># FYE678-10VL<\/b> (10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">10 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE678-80VL<\/b> (80 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;5 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">80 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE607-10VL<\/b> (10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"210\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">10 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE607-80VL<\/b> (80 preps) <\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">80 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE608-10VL<\/b> (10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;3 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">10 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE608-80VL<\/b> (80 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;3 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">80 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE609-10VL<\/b> (10 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>9<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">10 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE609-80VL<\/b> (80 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>9<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"110\">100 \u03bcl\/vial<\/td>\n<td align=\"right\" width=\"100\">80 vials<\/td>\n<\/tr>\n<tr>\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup> \u03bcg\/\u03bcl)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE607-96WL<\/b> (96 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>50 \u03bcl\/well<\/td>\n<td align=\"right\">96 wells<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE608-96WL<\/b> (96 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;3 x 10<sup>8<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>50 \u03bcl\/well<\/td>\n<td align=\"right\">96 wells<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><span><b># FYE609-96WL<\/b> (96 preps)<\/span><\/p>\n<div>\n<table border=\"0\" width=\"200\">\n<tbody>\n<tr>\n<td colspan=\"2\"><strong>Efficiency &gt;1 x 10<sup>9<\/sup> cfu\/\u03bcg<\/strong><\/td>\n<\/tr>\n<tr>\n<td>50 \u03bcl\/well<\/td>\n<td align=\"right\">96 wells<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<p>[\/et_pb_toggle][et_pb_toggle title=&#8221;Support\/ Documents&#8221; _builder_version=&#8221;4.16&#8243; title_level=&#8221;h4&#8243; title_font=&#8221;|700|||||||&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<div><\/div>\n<table width=\"1687\">\n<tbody>\n<tr>\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">Manual<\/span><\/strong><\/td>\n<td style=\"text-align: center;\"><strong><span style=\"color: #000000;\">ECOS Card<\/span><\/strong><\/td>\n<\/tr>\n<tr style=\"text-align: center;\">\n<td><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2023\/04\/ECOS-Manual-VerU0923-Reading-\u5df2\u58d3\u7e2e.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2535 aligncenter\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<td><a href=\"https:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/ECOS-CARD-FULL.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2535 aligncenter\" src=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" srcset=\"https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg 300w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-150x150.jpg 150w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-230x230.jpg 230w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-350x350.jpg 350w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-480x480.jpg 480w, https:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF.jpg 655w\" sizes=\"(max-width: 40px) 100vw, 40px\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/et_pb_toggle][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Description DH5\u03b1 is the most popular E. col [&hellip;]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"<div id=\"pl-1370\" class=\"panel-layout\">\r\n<div id=\"pg-1370-0\" class=\"panel-grid panel-no-style\" data-style=\"{\"cell_alignment\":\"flex-start\"}\">\r\n<div id=\"pgc-1370-0-0\" class=\"panel-grid-cell\" data-style=\"{\"background_display\":\"tile\",\"vertical_alignment\":\"auto\"}\" data-weight=\"0.61803399209206\">\r\n<div id=\"panel-1370-0-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"0\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n<div class=\"all_font\">\r\n<div class=\"all_font\">\r\n<div>\r\n\r\n<b style=\"font-family: inherit; font-size: 16px; font-style: inherit; letter-spacing: 0px;\">Strain: DH5\u03b1<\/b>\r\n\r\n<\/div>\r\n<div>\r\n<div>Description<\/div>\r\n<div>\r\n<div>DH5\u03b1 is the most popular\u00a0<em>E. coli<\/em>\u00a0strain for everyday cloning applications. It supports blue\/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5\u03b1 carries\u00a0<em>rec<\/em>A1 mutation that eliminates homologous recombination ensuring insert stability. It also carries\u00a0<em>end<\/em>A1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).<\/div>\r\n<\/div>\r\n<div>Genotype<\/div>\r\n<div>\r\n<div><em>end<\/em>A1\u00a0<em>rec<\/em>A1\u00a0<em>rel<\/em>A1\u00a0<em>gyr<\/em>A96\u00a0<em>hsd<\/em>R17(<em>rk<sup>-<\/sup>, mk<sup>+<\/sup><\/em>)\u00a0<em>pho<\/em>A\u00a0<em>sup<\/em>E44\u00a0<em>thi<\/em>-1 \u0394(<em>lac<\/em>ZYA-<em>arg<\/em>F)U169 \u03a680 \u0394(<em>lac<\/em>Z)M15 F<sup>-<\/sup><\/div>\r\n<\/div>\r\n<div><\/div>\r\n<div><\/div>\r\n<div>Features<\/div>\r\n<div>\r\n<div>Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.<\/div>\r\n<\/div>\r\n<div><\/div>\r\n<div>Applications<\/div>\r\n<div>\r\n<div>\r\n<ul>\r\n \t<li>Cloning and subcloning<\/li>\r\n \t<li>Scale-up application<\/li>\r\n \t<li>Blue\/white screening<\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<div>Efficiency<\/div>\r\n<div>\r\n<div>\r\n\r\n<strong>>1 x 10<sup>8<\/sup>\u00a0cfu\/ \u03bcg<\/strong>\r\nRecommended for:\r\n<ul>\r\n \t<li>Subcloning<\/li>\r\n \t<li>General cloning<\/li>\r\n<\/ul>\r\n<strong>>3 x 10<sup>8<\/sup>\u00a0cfu\/ \u03bcg<\/strong>\r\nRecommended for:\r\n<ul>\r\n \t<li>High-efficiency cloning<\/li>\r\n \t<li>Cloning low abundance transcripts<\/li>\r\n \t<li>General cloning<\/li>\r\n \t<li>Gene library<\/li>\r\n<\/ul>\r\n<strong>>1 x 10<sup>9<\/sup>\u00a0cfu\/ \u03bcg<\/strong>\r\nRecommended for:\r\n<ul>\r\n \t<li>Super high-efficiency cloning<\/li>\r\n \t<li>Cloning lowest abundance transcripts<\/li>\r\n \t<li>Gene library<\/li>\r\n<\/ul>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"all_font\"><\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<div id=\"pgc-1370-0-1\" class=\"panel-grid-cell\" data-weight=\"0.38196600790794\">\r\n<div id=\"panel-1370-0-1-0\" class=\"so-panel widget widget_sow-editor panel-first-child panel-last-child\" data-index=\"1\" data-style=\"{\"background_image_attachment\":false,\"background_display\":\"tile\"}\">\r\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\r\n<div class=\"siteorigin-widget-tinymce textwidget\">\r\n<div class=\"all_font\">Specification<\/div>\r\n<div class=\"all_font\">\r\n\r\n<span style=\"color: #333333;\">Store at<\/span>\r\n\r\n<img class=\"alignnone wp-image-2329\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/70-oC-300x222.png\" alt=\"\" width=\"97\" height=\"71\" \/>\r\n\r\n<span style=\"color: #333333;\"><b># FYE678-10VL<\/b>\u00a0(10 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >5 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">10 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE678-80VL<\/b>\u00a0(80 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >5 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">80 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE607-10VL<\/b>\u00a0(10 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"210\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">10 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE607-80VL<\/b>\u00a0(80 preps)\u00a0<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">80 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE608-10VL<\/b>\u00a0(10 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >3 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">10 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE608-80VL<\/b>\u00a0(80 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >3 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">80 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE609-10VL<\/b>\u00a0(10 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>9<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">10 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE609-80VL<\/b>\u00a0(80 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>9<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">100 \u03bcl\/vial<\/td>\r\n<td align=\"right\" width=\"100\">80 vials<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"110\">Control Plasmid (pUC19)<\/td>\r\n<td align=\"right\" width=\"100\">5 \u03bcl (10<sup>-4<\/sup>\u00a0\u03bcg\/\u03bcl)<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE607-96WL<\/b>\u00a0(96 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>50 \u03bcl\/well<\/td>\r\n<td align=\"right\">96 wells<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE608-96WL<\/b>\u00a0(96 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >3 x 10<sup>8<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>50 \u03bcl\/well<\/td>\r\n<td align=\"right\">96 wells<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<span style=\"color: #333333;\"><b># FYE609-96WL<\/b>\u00a0(96 preps)<\/span>\r\n<div>\r\n<table border=\"0\" width=\"200\">\r\n<tbody>\r\n<tr>\r\n<td colspan=\"2\"><strong>Efficiency >1 x 10<sup>9<\/sup>\u00a0cfu\/\u03bcg<\/strong><\/td>\r\n<\/tr>\r\n<tr>\r\n<td>50 \u03bcl\/well<\/td>\r\n<td align=\"right\">96 wells<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<table style=\"height: 55px;\" width=\"1687\">\r\n<tbody>\r\n<tr>\r\n<td style=\"text-align: center;\">Manual<\/td>\r\n<td style=\"text-align: center;\">ECOS Card<\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"text-align: center;\"><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/07\/ECOS-1-Minute-Transformation-Competent-Cells-DH5a-JM109-XL1-blue-10B-BL21-1.pdf\"><img class=\"wp-image-2535 aligncenter\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\r\n<td><a href=\"http:\/\/yeastern.com\/wp-content\/uploads\/2018\/05\/ECOS-CARD-FULL.pdf\"><img class=\"wp-image-2535 aligncenter\" src=\"http:\/\/yeastern.com\/wp-content\/uploads\/2017\/10\/PDF-300x300.jpg\" alt=\"\" width=\"40\" height=\"40\" \/><\/a><\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>\r\n<\/div>","_et_gb_content_width":"","footnotes":""},"_links":{"self":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1370"}],"collection":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/users\/20"}],"replies":[{"embeddable":true,"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1370"}],"version-history":[{"count":46,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1370\/revisions"}],"predecessor-version":[{"id":6879,"href":"https:\/\/yeastern.com\/index.php?rest_route=\/wp\/v2\/pages\/1370\/revisions\/6879"}],"wp:attachment":[{"href":"https:\/\/yeastern.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1370"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}