{"id":1366,"date":"2017-07-11T13:57:53","date_gmt":"2017-07-11T05:57:53","guid":{"rendered":"http:\/\/yeastern.com\/?page_id=1366"},"modified":"2024-05-31T15:44:17","modified_gmt":"2024-05-31T07:44:17","slug":"ecos-1-minute-transformation-competent-cells","status":"publish","type":"page","link":"https:\/\/yeastern.com\/?page_id=1366","title":{"rendered":"ECOS\u2122 Competent Cells"},"content":{"rendered":"

[et_pb_section fb_built=”1″ specialty=”on” _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_menu menu_id=”76″ active_link_color=”#000000″ _builder_version=”4.25.1″ menu_font=”|600|||||||” menu_text_color=”#474747″ menu_font_size=”15px” custom_margin=”||||false|false” custom_margin_tablet=”” custom_margin_phone=”” custom_margin_last_edited=”on|desktop” hover_enabled=”0″ global_colors_info=”{}” menu_text_shadow_style=”preset1″ sticky_enabled=”0″][\/et_pb_menu][\/et_pb_column][et_pb_column type=”3_4″ specialty_columns=”3″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_row_inner _builder_version=”4.16″ custom_margin=”|10px|-21px|||” custom_padding=”|0px|0px|0px||” global_colors_info=”{}”][et_pb_column_inner saved_specialty_column_type=”3_4″ _builder_version=”4.16″ global_colors_info=”{}”][et_pb_image src=”https:\/\/yeastern.com\/wp-content\/uploads\/2020\/02\/ecos.png” _builder_version=”4.16″ custom_padding=”|588px||||” global_colors_info=”{}”][\/et_pb_image][et_pb_post_title meta=”off” featured_image=”off” admin_label=”Post Title” _builder_version=”4.16″ title_font=”|700|||||||” title_font_size=”39px” custom_margin=”|-389px|30px|||” title_font_size_tablet=”” title_font_size_phone=”” title_font_size_last_edited=”on|desktop” text_shadow_style=”preset1″ global_colors_info=”{}”][\/et_pb_post_title][et_pb_text _builder_version=”4.16″ global_colors_info=”{}”]<\/p>\n

The fastest transforming cells worldwide- <\/strong><\/span><\/h1>\n

1 minute transformation<\/span><\/strong><\/span><\/h1>\n
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With “ECOS\u2122” standing for “E<\/em><\/strong>.\u00a0C<\/strong>oli\u00a0<\/em>O<\/strong>ne-S<\/strong>tep”, and its reverse “SOCE” standing for “SOC\u00a0<\/strong>E<\/strong>liminated”,\u00a0 the name itself speaks for the advantages.<\/div>\n
<\/strong><\/div>\n
1\u00a0<\/strong>minute,\u00a0<\/span>1<\/strong>\u00a0step high-efficiency transformation and no SOC recovery step required, truly a design to save your time.<\/span><\/div>\n<\/div>\n<\/div>\n

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Choose a strain<\/h2>\n

[\/et_pb_text][et_pb_toggle title=”Don’t know which strain to use? LOOK HERE!” icon_color=”#ffffff” open_icon_color=”#ffffff” _builder_version=”4.16″ title_text_color=”#ea9000″ title_level=”h4″ title_font=”Verdana|700|||||||” title_text_align=”center” title_font_size=”21px” custom_margin=”||0px||false|false” custom_padding=”5px||7px|0px|false|false” box_shadow_style=”preset1″ box_shadow_vertical=”0px” box_shadow_blur=”4px” box_shadow_color=”#e09900″ box_shadow_position=”inner” global_colors_info=”{}”]<\/p>\n

Selection Guide for ECOS Competent Cells<\/span><\/h4>\n
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Mutations and Respective Strains<\/span><\/h4>\n
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DH5\u03b1<\/strong><\/h1>\n

With its high efficiency, it is suitable for cloning large plasmids<\/strong><\/span> and cDNA library construction, also allowing for blue-white screening<\/span><\/p>\n

Use for<\/strong><\/p>\n

Cloning \/ Subcloning\u00a0<\/span><\/p>\n

Scale-up application<\/span><\/p>\n

Blue\/white screening<\/span><\/p>\n

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<\/strong><\/h3>\n

ECOS\u2122 X Competent Cells<\/strong><\/h3>\n

[DH5\u03b1]<\/strong><\/h3>\n

<\/strong><\/p>\n

<\/strong>\"\"<\/p>\n

\u00a0<\/strong><\/p>\n

Super High Efficiency<\/span><\/strong><\/p>\n

~5 x <\/span><\/strong>109<\/sup>\u00a0cfu\/\u03bcg<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/strong><\/span><\/p>\n

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<\/strong><\/h3>\n

ECOS\u2122 101 Competent Cells <\/strong><\/h3>\n

[DH5\u03b1]<\/strong><\/h3>\n

<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

<\/strong><\/p>\n

<\/strong><\/p>\n

<\/b><\/p>\n

High Efficiency<\/b><\/p>\n

One step, one tube, finish in 6 minutes !<\/strong><\/b><\/span><\/p>\n

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BL21(DE3)<\/strong><\/h1>\n

Lack of Lon<\/em> and Omp<\/em> proteases allows for high-level protein expression<\/strong><\/span><\/p>\n

\n<\/div>\n

Use for<\/strong><\/div>\n

IPTG induced gene expression of\u00a0 toxic\/non-toxic protein expression using T7-based vectors<\/p>\n

Subcloning<\/p>\n

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<\/strong><\/h3>\n

ECOS\u2122 21 Competent Cells [BL21(DE3)]<\/strong><\/h3>\n

<\/strong><\/p>\n

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\u00a0<\/strong><\/p>\n

High Levels of Protein Expression<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/span><\/span><\/strong><\/p>\n

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ECOS\u2122 Sonic\u2122 Competent Cells [BL21(DE3) Derived]<\/strong><\/h3>\n

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High Efficiency >10<\/span>8<\/sup>\u00a0cfu\/ug<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/span><\/strong>\u00a0\u00a0<\/strong><\/p>\n

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JM109<\/strong><\/h1>\n

The fastest growing strain; colonies show up about 8 hours<\/span><\/strong>. A<\/span>lso carries the F\u2019episome, which allows for blue\/white screening<\/span><\/p>\n

Use for<\/strong><\/span><\/p>\n

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Urgent needs cloning needs<\/p>\n

Preparation of ssDNA<\/p>\n

Gene library construction<\/p>\n

Subcloning<\/p>\n<\/div>\n

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ECOS\u2122 9-5 Competent Cells [JM109]<\/strong><\/h3>\n

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Rapid Growing\u00a0<\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/b><\/strong><\/span><\/p>\n

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DH10B<\/strong><\/h1>\n

A MC1061 derivative which is\u00a0<\/span>Streptomycin resistant and can prevent DNA methylation<\/p>\n

Designed to propagate large inserts<\/strong><\/span> in DNA library cloning<\/span><\/p>\n

Use for<\/strong><\/span><\/p>\n

Genomic library cloning<\/p>\n

Large inserts<\/p>\n

Cloning of methylated cytosine or adenine containing DNA<\/p>\n

Blue\/white screening<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_text_color=”#158701″ text_font_size=”18px” min_height=”494px” custom_padding=”||18px|||” link_option_url=”https:\/\/yeastern.com\/?page_id=1378″ border_radii=”on|10px|10px|10px|10px” border_width_all=”1px” border_color_all=”rgba(124,218,36,0.5)” global_colors_info=”{}”]<\/p>\n

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ECOS\u2122 10B Competent Cells [DH10B]<\/strong><\/h3>\n

<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

Allow Large Inserts<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/strong><\/span><\/p>\n

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XL-Blue<\/strong><\/h1>\n

A\u00a0Tetracycline resistant strain modified to be ideal for blue\/white screening<\/span><\/strong><\/p>\n

Use for<\/strong><\/p>\n<\/div>\n

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Blue\/white screening<\/p>\n

Cloning \/ Subcloning<\/p>\n

cDNA library<\/p>\n<\/div>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_text_color=”#158701″ text_font_size=”18px” custom_margin=”-3px||23px|||” custom_padding=”0px||27px|||” link_option_url=”https:\/\/yeastern.com\/?page_id=1374″ border_radii=”on|10px|10px|10px|10px” border_width_all=”1px” border_color_all=”rgba(124,218,36,0.5)” global_colors_info=”{}”]<\/p>\n

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ECOS\u2122 Blue Competent Cells [XL1-Blue]<\/strong><\/h3>\n

<\/strong><\/p>\n

\"\"<\/strong><\/p>\n

<\/span><\/strong><\/p>\n

Ideal for Blue\/white Screening\u00a0<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/b><\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_text_color=”#000000″ header_font_size=”35px” text_orientation=”center” global_colors_info=”{}”]<\/p>\n

GM2163<\/strong><\/h1>\n

A\u00a0<\/span>Chloramphenicol resistant K12 strain deficient in both\u00a0dam<\/em>\u00a0and\u00a0dcm<\/em>\u00a0genes<\/span><\/strong>,\u00a0for use with Dam or Dcm-sensitive restriction enzymes<\/span><\/p>\n

Use for\u00a0<\/strong><\/span><\/p>\n

Plasmids free of Dam and Dcm methylations<\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.16″ text_text_color=”#158701″ text_font_size=”18px” custom_margin=”185px|||||” custom_padding=”||45px|||” link_option_url=”https:\/\/yeastern.com\/?page_id=1380″ border_radii=”on|10px|10px|10px|10px” border_width_all=”1px” border_color_all=”rgba(124,218,36,0.5)” global_colors_info=”{}”]<\/p>\n

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ECOS\u2122 2163 Competent Cells [GM2163]<\/strong><\/h3>\n

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\"\"<\/strong><\/p>\n

No Methylations<\/span><\/strong><\/p>\n

One step, one tube, finish in 6 minutes !<\/strong><\/span><\/p>\n

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Yeastern Biotech Co., Ltd. owns the patent of ECOS\u2122 technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No.\u00a0US 6,864,088; GB 2383582; I 229696; FR 2832727<\/strong>, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS\u2122 chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS\u2122 protocol or its similar version, please contact our headquarters in Taiwan for licensing.<\/div>\n<\/div>\n<\/div>\n

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Each lot of competent cells has to pass three quality control tests before shipping:
<\/strong><\/div>\n
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  1. Efficiency test:<\/strong>\u00a0each batch of ECOS\u2122 should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.<\/li>\n
  2. Contamination test:<\/strong>\u00a0competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.<\/li>\n
  3. A-complementation test:<\/strong>\u00a0this test is performed for all ECOS\u2122 competent cells except for ECOS\u2122 21(DE3) and ECOS\u2122 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%<\/li>\n<\/ol>\n<\/div>\n

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    The fastest transforming cells worldwide- 1 minute tran […]<\/p>\n","protected":false},"author":20,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"

    \r\n
    \r\n\t
    Description<\/span><\/strong><\/div>
    Experience the world fastest transformation competent cells!\u00a0One\u00a0<\/strong>minute and\u00a0one<\/strong>\u00a0step high-efficiency transformation, no heat-shock needed and no SOC recovery step required!<\/div>
    ECOS\u2122 Competent Cells are the first innovative products of Yeastern Biotech\u2019s R&D team, which strives to bring noble products that make research faster and easier. ECOS\u2122 is Yeastern Biotech\u2019s registered trademark in USA, Japan, Canada, Korea, and Taiwan. The name speaks for the merits of this line of products -\u00a0E<\/em><\/strong>.\u00a0C<\/strong>oli\u00a0<\/em>O<\/strong>ne-S<\/strong>tep transformation. The reverse of this name is SOCE \u2013\u00a0SOC<\/strong>E<\/strong>liminated. ECOS\u2122 protocol allows users to finish the transformation within one step and to skip the SOC recovery step. Therefore, ECOS\u2122 competent cells are the fastest transforming cells worldwide. The traditional protocol requires recovery step and takes 1 ~ 2 hours to finish, but ECOS\u2122 allows transformation to be finished within 1 minute and the procedures are a lot simpler. Please check the ECOS\u2122 protocol section.<\/div>
    \u00a0<\/div>
    The latest progress in the ECOS\u2122 product section is the development of another 3 protocols in addition to the standard ECOS\u2122 protocol that was first introduced in 2003. The non-heat shock protocol allows transformation to be further simplified, and the 2 ~ 6-minute protocol enhances transformation efficiency significantly. These protocols have been developed based on the results of numerous experiments involving different antibiotics, plasmid size, and various conditions. This year, Yeastern Biotech offers our customers a new ECOS\u2122 competent cells with similar transformation efficiency to that of electroporation for applications which require very high transformation efficiency such as the construction of genomic libraries.<\/div><\/div>
    \u00a0<\/div>
    ECOS\u2122 Efficiency Guarantee<\/strong><\/div>
    ECOS\u2122 competent cells should be stored in a \u2013 70\u00b0C freezer. Since competent cells are very sensitive to freeze-thaw cycles, exposure to temperature variations should be minimized. In order to control the quality of competent cells, Yeastern Biotech adds electronic temperature monitors to record the temperature during the shipping process. Yeastern Biotech is the only company that offers this service.<\/div><\/div>
    \u00a0<\/div>
    ECOS\u2122 Technology is Patented<\/strong><\/div>
    Yeastern Biotech Co., Ltd. owns the patent of ECOS\u2122 technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No.\u00a0US 6,864,088; GB 2383582; I 229696; FR 2832727<\/strong>, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS\u2122 chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS\u2122 protocol or its similar version, please contact our headquarter in Taiwan for licensing.<\/div><\/div>
    \u00a0<\/div>
    Features<\/strong><\/div>
    • No LB\/SOC required<\/li>
    • Revolutionary 1- or 6-minute transformation, instead of 1 ~ 2 hours<\/li>
    • Procedures are simplified<\/li>
    • Several protocols to choose from according to your needs for efficiency and\u00a0 convenience<\/li>
    • Strict QC process to check the efficiency of each batch produced<\/li>
    • Ideal for bench users to automatize cloning projects<\/li>
    • Electronically monitored shipping process to check temperature fluctuation during shipping<\/li><\/ul><\/div><\/div>
      Quality Control<\/strong><\/div>

      Each lot of competent cells has to pass three quality control tests before shipping:<\/p>

      1. Efficiency test:<\/strong>\u00a0each batch of ECOS\u2122 should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.<\/li>
      2. Contamination test:<\/strong>\u00a0competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.<\/li>
      3. A-complementation test:<\/strong>\u00a0this test is performed for all ECOS\u2122 competent cells except for ECOS\u2122 21(DE3) and ECOS\u2122 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%.<\/li><\/ol><\/div><\/div>
        ECOS\u2122 Protocol Comparison Table<\/strong><\/div>
        \"\"<\/div>
        \u00a0<\/div>
        ECOS\u2122 Protocol<\/strong><\/div><\/div>
        \u00a0<\/div>
        \"\"<\/div>
        \"\"\u00a0<\/div>
        \u00a0<\/div>
        \u00a0<\/div>
        Strain's Features<\/strong><\/div><\/div>
        \u00a0\"\"<\/div>
        \u00a0<\/div>
        \"\"<\/div>
        \u00a0<\/div>
        \u00a0<\/div>

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